17426-153030赛默飞 Accucore PFP色谱

Noninvasive Human Nuclear Transfer with Embryonic Stem Cells

) (Falcon) Pipette (holding, 25-µm inner diameter, 120- to 150-µm outer diameter) (Humagen Fertility Diagnostics) Pipette (beveled, 13- to 15-µm inner diameter) (Humagen Fertility Diagnostics) Plates (tissue culture, 12-well)

利用纳升级液相色谱- 串联质谱进行蛋白质鉴定

。 电喷雾对无机盐非常敏感，因此肽段样品上样之前必须进行脱盐处理。最有效的一种方法是使用反相高效液相色谱法（RP- HPLC) 。反相高效液相色谱法不仅可以去除盐类 ，而且还可以纯化和浓缩肽段样品。这就是为什么液相色谱- MS/MS (LC-MS/MS)是蛋白质组学中最常见的配制。由于电喷雾技术跟样品浓度高度相关，色谱柱的直径是主要参数，减少柱子的直径可以提高灵敏度 [7] 。目前用直径 50~100 μm 的纳米级色谱柱来提高质谱仪进样口的肽段浓度。此外，利用纳米级色谱柱将流量减少到 150~300

Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applicati

‐ and closed‐ring AEAB derivatives at 300 µM on NHS‐derivatized slides. Antibodies were applied to the glycan array at the concentrations indicated in the figure, and detected with appropriate fluorescently labeled secondary antibodies (Song et al., ). The x