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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存
- 英文名:
Neutral Protease NB From Clostridium Histolyticum
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
N279017-80U
Description
Neutral Protease NB is chromatographically purified and contains an increased proteolytic activity. It is largely free from collagenolytic activity (PZ U) and the endotoxin level is very low.
Application
Neutral Protease NB is, mostly in combination with Collagenase NB 1 or another Collagenase NB product, suitable for cell isolation from various tissue types.
Specifications
Contains highly active, chromatographically purified neutral protease from Clostridium histolyticum.
Neutral protease activity (DMC): ≥ 4.00 U/mg.
Largely free of collagenolytic activity.
Endotoxin: ≤ 50 EU**/mg (Ph. Eur.)
Mr ca. 34 000 • CAS [9001-92-7]
References and Definitions
Unit definition:
*Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
**Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
References
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
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文献和实验) 式中:X——样品的酶活力,u/g(u/mL); A——试样溶液的平均吸光度; K——吸光常数; 8——反应试剂的总体积,mL; 2——吸取酶液2.00mL,以1min计; 1/10——反应时间10min,以1min计; n——稀释倍数 E——紫外法与福林法的换算系数(中性、碱性蛋白酶系数为0.50;酸性蛋白 酶系数为0.77)。 所得结果表示至整数。 7 结果的允许差
2F+DwTquooTcizAj6Sh40arj1e+iWJ2ispgsbT801bWi9OaaVDQ0u7N9Rf22Q7je3ypEkT/aXtAx/4gHh20RbetchuWXizve3YY/3L0LGHHqt8Cva+97zf/vEfvyYb4imWXpTxLz8m4rvgjodswc3X26Xnn2o3LbjZPv/Fv5GGuGa33/ZzO2n2HD17NAEWeUXwmQh44uy32U+uutL+69e+6jjedfsddvSxx3m8xNNFQ67wipqQKHyESZ35
) Ni 28 58.69 铌(niobium) Nb 41 92.00 氮(nitrogen) N 7 14.00
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