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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 4°C for short-term, -20°C for long-term. Avoid repeated freezing and thawing cycles.
- 保质期:
见COA
- 英文名:
GST440 Probe
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- 规格:
10 Tests; 20 Tests; Bulk

| 产品信息
品名:NebuProbe™ GST440 Probe
货号:NBPD-101768
产品分类:Bacteria / Virus Probes
规格:10 Tests; 20 Tests; Bulk
| 产品描述
NebuProbe™ GST440 Probe (Cat# NBPD-101768) is designed to detect the microbes: Group 1 clones (AC044 and AC051), closely related to Clostridium stercorarium
| 产品属性:
染色体定位:
标签:Green/Red/Aqua/Orange/DIG/BIO
特异性:
应用:These probes were specific when tested against a panel of nucleic acids from closely related bacteria.
同义词:FISH探针; 荧光原位杂交FISH探针; Bacteria / Virus Probes; 细菌/病毒探针; GST440 Probe; GST440探针; GST440 Probe探针; GST440 Probe;
储存条件:Store at 4°C for short-term, -20°C for long-term. Avoid repeated freezing and thawing cycles.
For Research Use Only!
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文献和实验一、GST pull-down 基本知识1. GST pull-down 概念GST pull-down:是利用重组技术将探针蛋白与 GST 谷胱苷肽巯基转移酶融合,融合蛋白通过 GST 与固相化在球珠上的 GSH 谷胱甘肽结合。从而分离出能与融合蛋白相互作用的蛋白的技术。2. GST pull-down 研究对象二、GST pull-down 原理1. GST pull-down 结合原理2. GST pull-down 分离原理三、GST pull-down 流程1. 蛋白的抽提与纯化:①
GST fusion protein purification GST融合蛋白纯化
resuspending on ice circa 10'after thawing.3)transfer to 50ml conical ss34 flip top tubes.4)add 10mg lysozyme powder to the 50ml (cells from 1 liter now in 1 50ml tube),digest 15' on ice.5)sonicate with large probe 1' 80% power,freeze in lN,thaw at37℃
GST fusion protein purification--GST融合蛋白纯化
GST fusion protein purification GST融合蛋白纯化John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze
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