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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Glutathione Magnetic Beads
- 保质期:
见COA
- 供应商:
北京辰辉创聚生物技术有限公司
- 保存条件:
2-8 °C; Freezing is prohibited

| 产品信息:
品名:NebuChem™ Glutathione Magnetic Beads
货号:NBGN-100128
品牌:辰辉创聚生物
规格:0.5ml;5ml;Bulk
| 产品描述:
NebuChem™ Glutathione Magnetic Beads(CAT#NBGN-100128) are developed for quick and efficient small‐scale purification of recombinant glutathione‐S‐transferase (GST)-tagged fusion proteins from bacteria, yeast and mammalian crude cell lysates. NebuChem™ Glutathione Magnetic Beads(CAT#NBGN-100128) are superparamagnetic beads pre-coupled with reduced glutathione (GSH). For purification, samples containing the target proteins are added to the Glutathione magnetic beads. The GST-tagged proteins bind to GSH on the magnetic beads. The beads are then washed and the GST-tagged proteins are eluted. Magnetic separation simplifies protein purification process by eliminating the need for centrifugation, minimizes sample loss and removes the excessive steps of a centrifugation-based purification process.
| 产品属性:
Storage:This product is stable until the expiration date stated on the COA, when stored unopened at 2–8°C. Freezing is prohibited the product. Keep the magnetic beads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Completely resuspend the beads before use. Observe sterile technique to avoid bacterial/fungal contamination.
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文献和实验而在无磁场时无磁性。7、不同的Dynabeads类型特殊的亲水和疏水特性能促进分子结合到它们的表面上。Dynal Biotech提供各种在其表面已包被或未包被有配基的磁珠。二、Dynabeads的大小在细胞分离和细胞修饰过程中,标准尺度的Dynabeads 磁珠是4.5 μm,可应用于各种样本(如全血、骨髓、白细胞层)的细胞分离。而2.8 μm的Dynabeads通常用于分子水平上,如DNA、RNA、蛋白质的分离等。Dynal Biotech拥有专门的技术来生产直径为1~10 μm的Dynabeads
[2] ✦ CUT&RUN 原理: 图 4:CUT&RUN 原理 [3] 利用连有刀豆蛋白 A 的磁珠(concanavalin A-coated magnetic beads)结合细胞。使用非离子去污剂洋地黄皂苷进行细胞膜通透。然后孵育靶蛋白(如转录因子, TF )的抗体和 Protein A-MNase 。抗体和 Protein A-MNase 能够通过核孔进入细胞核,MNase 通过 Protein A 和抗体的介导切割靶蛋白附近的 DNA 序列。 MNase 的活化需要 Ca
原理 GST 纯化系统是利用GST (glutathione-S-transferase )融合蛋白与固定的谷胱甘肽(GSH)通过硫键共价亲和,通过GSH交换洗脱的原理来进行纯化 。1ml树脂大约可结合5-8 mg融合蛋白,并可反复使用数次。试剂u IPTG(异丙基硫代-β-D-半乳糖苷) 2g IPTG 溶解入10ml 水,过滤除菌,分装,-20℃保存。u Lysis buffer (50ml)1)2.5ml 1 M Tris,pH8.0 2)0.1ml 0.5ml EDTA 3)0.292
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