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Millipore货号F7425-0.2mg抗-FLAG兔抗

[别名:抗-ddddk(抗-dykddddk)]上海睿安生物13611631389

价格￥6172.30

品牌Sigma-Aldrich
货号F7425-0.2mg
产地made in USA
更新日期2025年11月01日

上海睿安生物科技有限公司

12 年金牌会员

说明

一般描述

FLAG表位是一种带有肠激酶切割位点的八氨基酸蛋白。

兔抗-FLAG多克隆亲核抗体抗-FLAG可识别位于FLAG融合蛋白上的FLAG表位。该抗体可与N-末端、N末端-Met及C末端FLAG融合蛋白反应。

免疫原:FLAG；肽序列DYKDDDDK

应用

采用斑点印迹、免疫印迹、免疫沉淀和免疫细胞化学检测，该抗体可以识别N-末端或C-末端FLAG标记融合蛋白上的FLAG表位。
请访问我们的 FLAG® 应用门户网站，了解更多产品详情。

生化/生理作用:FLAG表位在融合蛋白的免疫亲和纯化中具有重要作用。

外形:含有1％牛血清白蛋白和15mM 叠(dié)氮化钠的0.01M磷酸盐缓冲盐水(pH7.4)中的溶液

制备说明:在可承受免疫肽的色谱柱上通过亲和色谱进行纯化。

其他说明:2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

常见问题

Is Product F7425, ANTI-FLAG^® from rabbit, a monoclonal or polyclonal?

F7425 is a polyclonal antibody from rabbit.
What is the concentration of Product F7425, ANTI-FLAG^® from rabbit?

The concentration is 0.9 mg protein per ml.
How much Product F7425, ANTI-FLAG^® from rabbit, should be used in western blotting and ELISA applications?

For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used. A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.
How to get rid of background when using Product F7465, ANTI-FLAG^® from rabbit,?

To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.
Is there any references for the use of Product F7465, ANTI-FLAG^® from rabbit, in immunohistochemistry or western blotting?

K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.
What are the positive controls that can be used with Product F7425, ANTI-FLAG^® from rabbit?

The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.
Which document(s) contains shelf-life or expiration date information for a given product?

If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?

The lot specific COA document can be found by entering the lot number above under the "Documents" section.

How do I find price and availability?

There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.

What is the Department of Transportation shipping information for this product?

Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

My question is not addressed here, how can I contact Technical Service for assistance?

Ask a Scientist here.

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Merck-Millipore货号F7425-0.2mg抗-FLAG兔抗[别名:抗-ddddk(抗-dykddddk)]上海睿安生物13611631389

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