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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
西宝生物
- 保存条件：
-20 C
- 库存：
1 Set
- 英文名：
FURA PE-3/AM
- 规格：
BIOCHEMICALS
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文献和实验nm和380 nm，发射光波长为500 nm处测定各管荧光强度；再向各管内先后加入20 μl的Triton X-100溶液和20 μl的EGTA [Ethylene glycolbis (2-aminoethyl-ether)-N,N,N',N'-tetraacetic acid ] 溶液后，分别测定荧光强度。根据公式 Ca2＋浓度=Kd×(R-Rmin)×Fb2/[(Rmax-R)×Ff2] 式中Kd为Ca2＋结合Fura-2的解离常数，R为34 0nm
Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
Use of Fura-2 in whole cell suspensions to measure changes in intracellular Ca2+ is probably one of the simplest, yet most widely used protocols described in this volume. Whole cell suspensions are loaded with Fura-2 and then placed
Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
, which cleaved the ester bond and left the calcium-sensitive free acid trapped within the cell (5 ). A number of improved calcium indicators have since been developed (e.g., fura-2, indo-1, and fluo-3), but the basic principles of dye loading and continuous
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