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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Fluorescent Transfect Reagent I (DOTAP:DOPE)
- 供应商:
上海西宝
- 规格:
10mg
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文献和实验Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
no. 18292-011) will be used in an attempt to transfect 2-3 day chicken embryos with a GFP retovirus. This patented lipid reagent binds DNA to form a complex which binds cells and allows for the subsequent uptake and expression of DNA. Although this reagent
Enhanced DNA transfer into fish bone cells using polyethylenimine
-to-transfect cells, in addition to a low cost, show that PEI is a reagent of choice for nonviral vector transfection.
ABSTRACT This protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal
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