SA26-10安捷伦Streptavidin-plus 10

Agilent公司毛细管柱简介

　　安捷伦公司的毛细管色谱柱自从Agilent公司合并了JW公司后其品质已达到世界一流水准, 就连它的价格也和DB系列完全一致。 HP-1－二甲基聚硅氧烷柱 说明：这是最常用的非极性键合固定相，HP-1（二甲基聚硅氧烷），具有极好的热稳定性并且在高温下流失很小，具有低的检测限      相似的固定相：DB-1,Rtx-1,SPB-1,CP Sil 5CB,MDN-1,DB-1h.t.,AT-1 007-1      恒温/程序升温温度范围：-60至325/350℃,-60

Nonradioactive Labeling and lmmunodetection of Recombinant Proteins

because of the extremely high dissociation constant for the biotin-avidin or biotin-streptavidin interaction. The dissociation constant is 10−15 /M (1 ). Erther streptavidin or avidin coqugates can be employed as biotin binding reagents but lower backgrounds

DETECTION OF RECOMBINANT PROTEINS BY ELISA

of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie