- ￥8252.39
- Agilent
- G1364-84523
- 2025年07月14日
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文献和实验M 20% sucrose 10g 50mM NaCl 500μL 5M 10mM EDTA 1mL 0.5M 10 mg/ml lysozyme 0.5g Note: Prepare the 50ml without lysozyme and than add
Small scale His-Tag fusion protein purification under denaturative conditions
enough Perform parallel purification procedures where you include 10, 20, 30, 40 or 50mM imidazole in the lysis, binding and washing buffer. Elute directly with 3x100ul elution buffer + 250mM Imidazol. Check eluted proteins on PAGE-SDS. Expect
Hints and precautions for the care, feeding and breeding of Neurospora
, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination
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