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文献和实验Using Cell‐ID 1.4 with R for Microscope‐Based Cytometry
are open?source. Curr. Protoc. Mol. Biol. 100:14.18.1?14.18.26. © 2012 by John Wiley & Sons, Inc. Keywords: image processing; fluorescence microscopy; Cell?ID; R
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
B. Culture/expand cells. We have obtained excellent results with 2 x 107 cells per library transfection. C. Trypsinize cells that are at >80% confluency, and transfer 2 x 107 cells to a 15 ml sterile screw topped tubes.
Construction and Characterization of Adenovirus Vectors
) Tubes (polystyrene, conical, 15-mL) Tubes (polystyrene, round-bottom, 5-mL) Tubes (ultracentrifuge, Quick-Seal, 16- x 76-mm) (Beckman 344322) Tubes (ultracentrifuge, 14- x 89-mm) Ultracentrifuge Vortexer Water baths
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