8003-0488安捷伦Sample Ferrule, Cr

Chromatin Immunoprecipitation (ChIP)

before and after heat shock, followed by shearing or restriction enzyme digestion of the DNA. Immunoprecipitation of cross-linked protein-DNA complexes containing histone H4 or RNA polymerase II was then carried out using specific antibodies. The covalent cross

Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)

capacity and a loading volume of up to 800 µl. Fused-silica capillary, 100 µm I.D. x 365 µm O.D. (Agilent Technologies or Polymicro Technologies) Fused-silica capillary (size and length depend on the desired split flow rate, see Step 10) Gold

Linear amplification of limiting amounts of RNA for gene expression studies

, 1µl RNAsin, 1µl Superscript II RT; Add 9µl mix to the aRNA/primer sample and incubate at 42°C for 1 hour; Add 3µl of RNAseH (removal of DNA/RNA hybrids); Incubate for 30 minutes at 37°C. Second strand synthesis