5500-1302安捷伦Capillary ST 0.12x

ChIP-Chip E. coli

Technology (ogt) provides good arrays in 4*44k format compatibel with Agilent scanner and hybridisation equipment [5] Hybridisation buffer materials: 5M Sodium chloride Sigma (S6316) 12x MES prepared as follows: 3.52g MES

Preparation of BAC DNA with by Alkaline Prep Method

until the pellet is re-suspended. 4. After all the pellets have been re-suspended (go back to 1st bottle, re-pipette 1-2x to look for clumps), move the bottles from the ice to the bench top. Let them sit for 5 minutes. Solution II: 0.2 M NaOH, 1% SDS (250 ml

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6). 5. S-gal LB powder (Sigma, St. Louis, MO) (see Note 7). 6. Solution I: 50 mM glucose, 25 mM Tris–HCl (pH 8.0), and10 mM EDTA (pH 8.0). Filter sterilized. Store at 4°C. 7. Solution II: 0.1 M NaOH and 1% SDS. Store at 4°C. 8. Solution