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- Agilent
- 5191-5911
- 2025年12月23日
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文献和实验in the hybridization bag, but may result in higher background. Discard wash 1 and add 1000 mL prewarmed wash 2 and incubate at 65 for 30 min. Discard wash 2 and add 1000 mL prewarmed wash 3 and incubate at 65 for 30 min.18. Air dry the filter on 3MM paper
Analys of Genomic DNA by Southern Hybridization
according to size by electrophoresis through an agarose gel. The DNA is then denatured in situ and transferred from the gel to a solid support (usually nitrocellulose filter or nylon membrane). The relative positions of the DNA fragments are preserved
Analys of Genomic DNA by Southe
blots in 50% formamide, 6 x SSPE for 30 minutes at 65℃. ( for 50 ml: 25 ml deionized formamide, 15 ml 20 x SSPE, 10 ml dH2O) Rinse in 2 x SSPE, remove excess liquid by dabbing the blot on 3MM paper. Wrap in Saran wrap and store. Expose overnight
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