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文献和实验protocol. Briefl y, disassociate cells from theplate, harvest by spin down the cell suspension at 250 × g for 5 min. Wash cell pellet with 500 μl of PBS and then resuspendedin QuickExtract solution. We typically use 50 μl for one well ina 24-well plate
Procedures for In Vitro Production of Bovine Embryos
1. Prepare embryo culture medium (modified KSOM) at least 2 h before removing embryos/oocytes from the fertilization drops.2. Make enough 50 ml microdrops of culture medium (30 oocytes/embryos per drop) on 60 x 15 mm petri dishes and cover with mineral oil
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
in microfuge. Aliquot about 450ul to a fresh tube. Freeze in lN2 and store -80C. Fluorometric GUS Mix pr assay 125u1 2 x MUG amd 50u1 100% Methanol prewarm at 37C and at t0 add 75ul extract, mix. When all have been done (up to 50) take out 50
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