5190-8756安捷伦Lanthanum 1000 ug/

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

Protocol for vacuum-infiltration transformation of Arabidopsis thaliana

the herbicide solution on the plantlets. Efficient recovery was achieved using the following protocol : seeds (up to 1 g : approximately 50,000 seeds) are mixed with 4-5 volumes of fine sand and sown on 30 cm X 30 cm trays containing medium sand. The trays

Loss of Heterozygosity

Boric Acid 0.02M Disodium EDTA ) (Advanced Biotechnologies)Acrylease (Stratagene)Glass cleaner (e.g., Windex, Glass Plus)95% ethanol2. EquipmentThermal cycler (MJ Research)Sequencing gel electrophoresis apparatus (Gibco BRL)High voltage power supplyGel