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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
Wavel Plate, ? Wave Retardation, 400-700nm Newport 10RP52-1 1 Rotation Stage, 1" Aperature Newport
(direct repeats) interspaced by short stretches of non- repetitive sequences (spacers,30 bp each). Each spacer is typically derived from foreign genetic material (protospacer),and directs the specifi city of CRISPR- mediated nucleic acid cleavage
plate and Cylcleseal PCR plate sealer (e.g. #1038-50-0 and #1044-39-4, Robbins Scientific Corp. Sunnyvale, CA) household one-gallon sealable storage bags (e.g. Glad Lock) heat sealable storage bags and heat sealer 0.2mm Sterile Filtration unit
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