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文献和实验Co. 07596-20 Pump-Drive (Masterflex L/S variable-speed economy drive) Cole Parmer Instrument Co. 7524-40
on how many blots are being hybridized. For small tube use 10-20ml prehybridization buffer. Note: Work behind an acrylic shield for all following procedures. 5. Remove [32P] dCTP from freezer and place behind shield in biological cabinet. 6. Prepare Initial
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
behind, the aqueous phase can then be pipetted up and placed in a clean, 1.5ml microtube. (6) Remove primers and dNTPs from the PCR reaction using Promega's "Wizard PCR preps". add 100µl of "Direct Purification Buffer
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