- ￥33770.34
- Thermo Fisher
- 042191
- 2025年08月27日
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
tip) alternatively: Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade); 9'' Pasteur pipettes; 70% Ethanol; Alcohol burner or Bunsen burner; Sterile plastic Petri dishes (100 x 15 mm); Sterile
Cool growth competent E . coli
2O; add 10 ml 250 mM KCl; pH to 7.0 with NaOH Adjust vol to 1 liter; Autoclave. Just before use, add 5 ml sterile 2M MgCl2 per liter TB per liter 10 mM PIPES Na salt 3.35 g 15 mM CaCl2 (Fisher) 2.2 g 250 mM KCl (Aldrich) 18.64g 55
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