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文献和实验4. Remove supernatant and keep (this will contain everything except large plasma membrane pieces, DNA, nucleoli), extract out 10µl for Bradford assay. 5. On ice, resuspend pellet in 374µl of Buffer C and add 26µl of 4.6M NaCl to give 300mM
0.1ml ethidium bromide (10mg/ml). [It may be useful at this point to spin down any precipitate that has formed. This can interfere with the banding process later.] Transfer to ultracentrifuge tube (1/2 X 2 inches (13 X 51 mm) quick seal
0.348g Isopropanol 20ml Retinoic acid all trans-Retinoic acid, Tretinoin, light-sensitive, (store at -20o C): Conc. Stock 16.6ml C20 H28 O2 10mM 300.4g/M 50mg EtOH > 96% 16
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