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- Thermo Fisher
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- 2025年08月15日
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
containing the antibody/agarose complex. Mix by flicking tube gently. (36) Incubate at RT for 15 min. (37) Pellet agarose by brief centrifugation (3000-5000 x g (6600 rpm) for 1 min), and collect supernatant into new tubes. (38) Repeat steps 35
/M 55.03g EDTA, pH 8.0 2mM 20mM 500mM 40ml 43.84g H2 O mQ 863.3ml 10x stock solution is stable at NT after 0.4µm filtration; for PAAG use as 1x, for agarose gel - as 0.5x; TBE is used in agarose gels
P. Use of denaturing gradient gel electrophoresis to study conformational transitions in nucleic acids [J]. Methods Enzymol, 1992, 212: 71-104.[35]Aggerholm A, Guldberg P, Hokland M, et al. Extensive intra- and interindividual heterogeneity of p15INK
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