60201-393赛默飞DEACTIVATED TUBING

一篇搞定 IP、co-IP 实验

或者互作蛋白复合物（红色+黄色）洗脱 Step 5：分析检测—Western Blot 或者质谱 常用“黑话” 以上示例图证明了 EDR1 和 EDR4 有相互作用。如果想要设计好实验，先清楚以下常用“黑话”含义。 阳性对照：证实细胞裂解物中确实存在靶蛋白（IP）或者互作蛋白对（co-IP，比如诱饵蛋白X和靶蛋白 Y），即为常说的 input。可直接取抽好的蛋白溶液进行 Western。 阴性对照：用来排除假阳性。IP/co-IP 后靶蛋白或者互作蛋白对都检测到了，固然是值得高兴

Purification of mAb (IgG)

7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

from the reservoir).     5)      Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head