063653赛默飞ProPac HIC-10 分析柱 (2.

Karyotyping of X. tropicalis

, a pair of frogs were mated (see Grainger Lab X. tropicalis Mating Protocol for details) and the resultant tadpoles were used in the following karyotyping protocol. 2. Place 10 Nieuwkoop & Faber Stage 26-34 tadpoles into a dish of deionized, distilled

原装Thermo SCX－固相萃取柱应用实例

原装Thermo－SCX－固相萃取柱/ 3ml / 60mg 应用实例： (1)对乙酰氨基酚――萃取方法： 样品前处理： 准确称取10mg对乙酰氨基酚移至100mL容量瓶中并用水定容至刻度，得到浓度为100ppm的溶液，取25mL醋氨酚溶液(100ppm)与25mL1%磷酸水溶液在50mL烧杯中混合。 柱活化：    依次2mL甲醇、2mL水淋洗。 样品载样：  每分钟进样1-2mL 冲洗：       2mL 的甲醇/水 (5/95， v/v) 洗脱：       2mL 甲醇

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

1.5 ml    1.2 mM EDTA 0.5M stock 120 µl    1.1% Triton X-100 10% stock 5.5 ml    0.01% SDS 10% stock 50 µl