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- 2025年07月11日
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文献和实验4 and 5 two more times for a total of 4 washes Add 10 ml 2x NETS and store at 4°C Prep cellulose the day before you need it mRNA prep Dilute 1 mg total RNA to 1 ml with 10mM Tris pH7.4 in c/f tube In a 2 ml BioRad mini column(#731-1550
I buffer (Tris pH8.4, 20 mM MgCl2 , 500 mM KCl) 5 µL DNAse I (RNAse free) q.s. 100 µL with DEPC H2 O Incubate at room temp. x10 min. Immediately proceed to next step. RNeasy Mini Prep Procedure
. Solutions and reagents: 100g P-11 cellulose phosphate fibrous cation exchanger (Whatman Inc, Clifton NJ) 6L 0.1M HCl 6L 0.1 M NaOH 2L 0.1 M MgSO4 2L 10x Column Buffer ~10 L 1x column buffer 10 M NaOH 3 fresh ( Equipment: Waring Blender
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