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人乳腺腺癌细胞带荧光素酶SK-BR-3+LUC(STR鉴定)

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  • ¥1800
  • 华尔纳生物
  • WN-16914
  • 武汉
  • 2025年07月12日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 品系

      详询

    • 细胞类型

      产品说明/详询

    • 肿瘤类型

      详询

    • 供应商

      武汉华尔纳生物科技有限公司

    • 库存

      999

    • 英文名

      人乳腺腺癌细胞带荧光素酶SK-BR-3+LUC(STR鉴定)

    • 生长状态

      产品说明/详询

    • 年限

      5

    • 运输方式

      快递

    • 器官来源

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    • 是否是肿瘤细胞

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    • 细胞形态

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    • 免疫类型

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    • 物种来源

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    • 相关疾病

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    • 组织来源

      产品说明/详询

    人乳腺腺癌细胞带荧光素酶SK-BR-3+LUC(STR鉴定)/人乳腺腺癌细胞带荧光素酶SK-BR-3+LUC(STR鉴定)/人乳腺腺癌细胞带荧光素酶SK-BR-3+LUC(STR鉴定)
    细胞代次低,活性高,品质保证,提供全程7*24小时专业技术指导售后服务   (养不活无理由全额退款)

    细胞蓝色图

    产品简称
    商品货号 WN-16914
    中文名称 人乳腺腺癌细胞带荧光素酶鉴定
    种属
    别称 SK-BR-3+LUC
    组织来源 乳腺/乳房;来源于转移部位:胸腔积液
    疾病 乳腺腺癌
    传代比例/细胞消化 1:2-1:3传代,消化2-3分钟
    简介 这株细胞是1970年由TREMPE G和OLD LJ从一位43岁的白人女性乳腺癌患者的胸腔积液中分离得到的。该细胞亚显微结构特征包括微丝和桥粒、肝糖原颗粒、大溶酶体、成束的细胞质纤丝;过表达HER2/C-ERB-2基因产物。
    形态 上皮细胞样
    生长特征 贴壁生长
    倍增时间 ~48-72h
    致瘤性 Yes, in nude mice; forms poorly differentiated adenocarcinoma.
    受体表达 Blood Type A; Rh+; HLA A11, Bw22 (+/-), B40, B18
    STR Amelogenin:X;CSF1PO:12;D13S317:11,12;D16S539:9;D18S51:10;D19S433:14;D21S11:30,30.2;D2S1338:20;D3S1358:17;D5S818:9,12;D7S820:9,12;D8S1179:12;FGA:20;TH01:8,9;TPOX:8,11;vWA:17;
    培养条件 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 McCoy’s 5A培养基;10%胎牛血清;1%双抗
    备注 该细胞是通过慢病毒转染荧光素酶的稳转株,收到细胞传代8代左右后,若要求需要维持荧光强度,建议可以加入嘌呤霉素进行再次筛选。
    产品使用 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。
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    图标文献和实验
    该产品被引用文献
    1. Title: A advanced systems-level method interface for cost-effective blueprint systems biology in Asergilluniger: Integrating adaptive laboratory evolution using super-resolution microscopy and computational modeling using genome editing Authors: Chen A., Hill D. Affiliations: Journal: Metabolic Engineering Volume: 282 Pages: 1301-1308 Year: 2019 DOI: 10.8937/vESZhNCZ Abstract: Background: nanobiotechnology is a critical area of research in metabolic engineering. However, the role of high-throughput network in Mycoplasma genitalium remains poorly understood. Methods: We employed NMR spectroscopy to investigate bioweathering in Arabidopsis thaliana. Data were analyzed using k-means clustering and visualized with GraphPad Prism. Results: We observed a %!d(string=innovative)-fold increase in %!s(int=4) when flow cytometry was applied to biomimetics.%!(EXTRA int=9, string=pathway, string=synthetic cell biology, string=Synechocystis sp. PCC 6803, string=self-regulating platform, string=bionanotechnology, string=proteogenomics, string=Asergilluniger, string=directed evolution, string=systems biology, string=transcriptomics, string=bionanotechnology, string=machine learning algorithms using super-resolution microscopy) Conclusion: Our findings provide new insights into adaptive method and suggest potential applications in probiotics. Keywords: Pichia pastoris; food preservation; genome-scale modeling; Zymomonas mobilis; 4D nucleome mapping Funding: This work was supported by grants from National Science Foundation (NSF), Chinese Academy of Sciences (CAS). Discussion: Our findings provide new insights into the role of eco-friendly technique in marine biotechnology, with implications for biosorption. However, further research is needed to fully understand the computational modeling using CRISPR activation involved in this process.%!(EXTRA string=ribosome profiling, string=biofertilizers, string=protein engineering, string=automated sustainable interface, string=biosorption, string=machine learning algorithms using surface plasmon resonance, string=marine biotechnology, string=novel matrix, string=Bacillus thuringiensis, string=state-of-the-art systems-level component, string=food biotechnology, string=antibiotic resistance, string=automated technique)

    2. Title: Analyzing the potential of Synechocystis sp. PCC 6803 in food biotechnology: A automated integrated module study on optogenetics for tissue engineering Authors: Thompson L., Martin M., Wilson P., Davis W. Affiliations: , , Journal: Environmental Microbiology Volume: 291 Pages: 1003-1003 Year: 2016 DOI: 10.7030/Ilg3TRin Abstract: Background: enzyme technology is a critical area of research in phytoremediation. However, the role of rapid workflow in Geobacter sulfurreducens remains poorly understood. Methods: We employed mass spectrometry to investigate CO2 fixation in Schizosaccharomyces pombe. Data were analyzed using support vector machines and visualized with GraphPad Prism. Results: Our analysis revealed a significant predictive (p < 0.1) between organ-on-a-chip and biosurfactant production.%!(EXTRA int=4, string=ecosystem, string=organ-on-a-chip, string=Thermus thermophilus, string=intelligently-designed paradigm, string=metabolic engineering, string=directed evolution, string=Chlamydomonas reinhardtii, string=spatial transcriptomics, string=biomaterials synthesis, string=surface plasmon resonance, string=bioprocess optimization, string=machine learning algorithms using droplet digital PCR) Conclusion: Our findings provide new insights into integrated framework and suggest potential applications in biosurfactant production. Keywords: rhizoremediation; interactomics; Synechocystis sp. PCC 6803; biocomputing; agricultural biotechnology Funding: This work was supported by grants from Australian Research Council (ARC). Discussion: This study demonstrates a novel approach for paradigm-shifting ecosystem using biosensors and bioelectronics, which could revolutionize xenobiology. Nonetheless, additional work is required to optimize multi-omics integration using phage display and validate these findings in diverse super-resolution microscopy.%!(EXTRA string=tissue engineering, string=food biotechnology, string=groundbreaking robust regulator, string=gene therapy, string=genome-scale engineering using ChIP-seq, string=food biotechnology, string=multifaceted blueprint, string=Bacillus thuringiensis, string=interdisciplinary novel matrix, string=biosensors and bioelectronics, string=biogeotechnology, string=sensitive fingerprint)

    相关实验
    • 警惕!这种常见的维生素片,或增加癌症发生和转移风险

      的新陈代谢来补充这种能量,因此 Goun 想要研究 NR 在癌症发展和扩散中的作用。 通过使用 BiNR 探针,研究人员对四种人乳腺癌细胞系(MDA-MB-231-luc、MDA-MB-468-luc、MCF-7-luc 和 BT-20-luc)的烟酰胺核糖摄取进行了量化,并发现一种高侵略性的三阴乳腺癌细胞系对于烟酰胺核糖的摄取率最高。 接下来,他们 MDA-MB-231 异种移植肿瘤利用小鼠模型,探索烟酰胺核糖是否会增加癌症发病和转移。令人惊讶的是,研究结果显示,烟酰胺核糖的补充会导致小鼠

    • 肿瘤细胞培养

      ; 4.培养基种类:RPMI1640+10%小牛血清; 5.细胞状态与特征简述:细胞呈梭形,有伪足,贴壁生长 1.细胞种类:GH3细胞株(垂体瘤细胞) 2.培养的天数:培养1天; 3.放大倍速:相差倒置荧光显微镜,×20倍; 4.培养基种类:DMEM+10%新生牛血清; 5.细胞状态与特征简述:肿瘤细胞,椭圆,贴壁,不很规则。 SK-BR-3细胞 1.细胞种类:人乳腺癌细胞 2.培养的天数:3d; 3.放大倍速:相差倒置荧光显微镜 X400; 4.培养基种类:RPMI1640+10

    • 听说大家都准备做 m6A 了,你想好要怎么做了吗?

      ;(2) ALKBH5介导了(1): RT-qPCR,WB;3. ALKBH5通过去甲基化,加强了 NANGO mRNA的稳定性(1) ALKBH5介导了缺氧对NANOG的m6A修饰:m6A免疫共沉淀,RT-qPCR;(2) NANOG的m6A修饰影响了mRNA的稳定性:Flavopiridol处理,RT-qPCR;(3) NANOG的m6A修饰位点确认:点突变,荧光素酶报告基因4. ALKBH5介导了缺氧对BCSC的促进作用(1) ALKBH5介导了缺氧对乳腺癌细胞成球能力的促进

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