Recombinant-Mouse-ATP-binding-
cassette-sub-family-D-member-3Abcd3ATP-binding cassette sub-family D member 3 Alternative name(s): 68 kDa peroxisomal membrane protein; PMP68 70 kDa peroxisomal membrane protein; PMP70- ￥14644
- CUSABIO
- CN
- CSB-CF001074MO
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
"-20°C/-80°C"
- 保质期：
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名：
Customized Mouse Abcd3 Protein (in vitro E.coli)
- 库存：
200
- 供应商：
武汉华美生物工程有限公司
- 规格：
20ug
Alternative Name(s)
ATP-binding cassette sub-family D member 3 Alternative name(s): 68 kDa peroxisomal membrane protein; PMP68 70 kDa peroxisomal membrane protein; PMP70Editorial/Sponsord
EditorialUniprot ID
P55096Gene Names
Abcd3Organism
MouseAASequence
MAAFSKYLTARNTSLAGAAFLLLCLLHKRRRALGLHGKKSGKPPLQNNEKEGKKERAVVD KVFLSRLSQILKIMVPRTFCKETGYLLLIAVMLVSRTYCDVWMIQNGTLIESGIIGRSSK DFKRYLFNFIAAMPLISLVNNFLKYGLNELKLCFRVRLTRYLYEEYLQAFTYYKMGNLDN RIANPDQLLTQDVEKFCNSVVDLYSNLSKPFLDIVLYIFKLTSAIGAQGPASMMAYLLVS GLFLTRLRRPIGKMTIMEQKYEGEYRYVNSRLITNSEEIAFYNGNKREKQTIHSVFRKLV EHLHNFIFFRFSMGFIDSIIAKYVATVVGYLVVSRPFLDLAHPRHLHSTHSELLEDYYQS GRMLLRMSQALGRIVLAGREMTRLAGFTARITELMQVLKDLNHGRYERTMVSQQEKGIEG AQASPLVPGAGEIINTDNIIKFDHVPLATPNGDILIQDLSFEVRSGANVLICGPNGCGKS SLFRVLGELWPLFGGRLTKPERGKLFYVPQRPYMTLGTLRDQVIYPDGKEDQKKRGISDQ VLKEYLDNVQLGHILEREGGWDSVQDWMDVLSGGEKQRMAMARLFYHKPQFAILDECTSA VSVDVEDYIYSHCRKVGITLFTVSHRKSLWKHHEYYLHMDGRGNYEFKKITEDTVEFGSExpression Region
1-659aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
2092Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动，请以官网信息为准
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文献和实验**产品信息可能有变动，请以官网信息为准
functions [ 16 – 18 ]. These studies demonstrated that Cas9 is a RNA-guided nuclease that is capable of binding to a targetDNA and introduce a double strand break in a sequencespecific manner, and its specifi city is determined by sequenceencoded
CRISPR/Cas9 是一种强大的基因组编辑工具，今天我们介绍 CRISPR/Cas9 系统操作。以下操作说明是基于作者冰糖个人实验室操作，仅供参考。 质粒介绍我们使用的是 Feng Zhang 实验室的系统的 pX330 质粒，如下图所示： 酶切系统 37 ℃, 3 hr；Run 1% gel，回收。Elute 到 50 mL H2O。我们利用 BbsI 消化载体形成粘末端，以利于下游 oligo 退火产物的连接；酶切时请充分，且绝对不能加 CIP 处理
选育新方法。而 CRISPR-Cas9 技术的出现，更是极大推动了微生物基因组编辑准确性和效率的提高。【背景】泓迅科技利用 CRISPR-Cas9 技术编辑酵母菌中的一个 920bp 的 ADE1 基因。ADE1 是腺嘌呤合成相关基因，如果 ADE1 失活，则细胞将会积累红色色素，呈现红色菌落。【方法】1、质粒构建2、sgRNA 活性检测3、挑选活性较高的 sgRNA 进行基因组定点编辑4、利用基因功能变化、表性变化和测序等方法检测基因定点修饰结果。【结论】1、从颜色表型变化得知，酵母菌落变成红色菌落，可知 ADE
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