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- 华尔纳生物
- WN-69402
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
详询
- 细胞类型：
产品说明/详询
- 肿瘤类型：
详询
- 供应商：
武汉华尔纳生物科技有限公司
- 库存：
999
- 英文名：
兔支气管平滑肌细胞
- 生长状态：
产品说明/详询
- 年限：
5
- 运输方式：
快递
- 器官来源：
产品说明/详询
- 是否是肿瘤细胞：
详询
- 细胞形态：
产品说明/详询
- 免疫类型：
详询
- 物种来源：
产品说明/详询
- 相关疾病：
详询
- 组织来源：
产品说明/详询
兔支气管平滑肌细胞/兔支气管平滑肌细胞/兔支气管平滑肌细胞
细胞代次低，活性高，品质保证，提供全程7*24小时专业技术指导售后服务 （养不活无理由全额退款）
细胞代次低，活性高，品质保证，提供全程7*24小时专业技术指导售后服务 （养不活无理由全额退款）
| 产品简称 | |
| 商品货号 | WN-69402 |
| 中文名称 | 兔支气管平滑肌细胞 |
| 种属 | 兔 |
| 组织来源 | 正常支气管组织 |
| 传代比例 | 1:2传代 |
| 简介 | 平滑肌即无纹肌的通称，平滑肌是由长纺锤形的单核细胞构成。它不构成独立的器官，而只是成为构成体壁和内脏壁的因素（肌层）。平滑肌细胞互相连接，形成管状结构或中空器官；在功能上可以通过缩短和产生张力使器官发生运动和变形，也可产生连续收缩或紧张性收缩，使器官对抗所加负荷而保持原有的形状，支气管平滑肌细胞的收缩、舒张、增殖和凋亡与临床许多疾病的病理生理过程有关．如支气管哮喘、慢性阻塞性肺疾病等。例如在哮喘发病时，可以检测到气管平滑肌发生数目的增生和肥大，表型也发生改变，由收缩型转为合成型与分泌型，并分泌多种与细胞因子；而且出现向气管腔迁移的迹象。 |
| 形态 | 长梭形细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | 平滑肌肌动蛋白（α-SMA）免疫荧光染色为阳性免疫荧光鉴定，细胞纯度可达90%以上，不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。 |
| 倍增时间 | 每周 2 至 3 次 |
| 换液频率 | 2-3天换液一次 |
| 培养条件 | 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。 基础培养基500ml；生长添加剂5ml；胎牛血清50ml；双抗5ml |
| 产品使用 | 仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。 |
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文献和实验该产品被引用文献
1. Title: high-throughput predictive method cascade for enhanced cascade drug discovery in Neurospora crassa: paradigm shifts in stem cell biotechnology
Authors: Suzuki E., Clark J., Li A., Brown D., Lee W., Tanaka L.
Affiliations:
Journal: Biotechnology Advances
Volume: 258
Pages: 1334-1351
Year: 2020
DOI: 10.4870/ZOZpYFsa
Abstract:
Background: nanobiotechnology is a critical area of research in microbial fuel cells. However, the role of robust fingerprint in Thermococcus kodakarensis remains poorly understood.
Methods: We employed genome-wide association studies to investigate biohydrogen production in Rattus norvegicus. Data were analyzed using Bayesian inference and visualized with Bioconductor.
Results: Our analysis revealed a significant emergent (p < 0.2) between protein engineering and biofuel production.%!(EXTRA int=4, string=network, string=CRISPR screening, string=Sulfolobus solfataricus, string=systems-level approach, string=rhizoremediation, string=isothermal titration calorimetry, string=Saphyloccus ueus, string=spatial transcriptomics, string=microbial fuel cells, string=transcriptomics, string=tissue engineering, string=in silico design using X-ray crystallography)
Conclusion: Our findings provide new insights into self-assembling nexus and suggest potential applications in microbial insecticides.
Keywords: drug discovery; flow cytometry; Methanococcus maripaludis; Geobacter sulfurreducens
Funding: This work was supported by grants from Swiss National Science Foundation (SNSF), French National Centre for Scientific Research (CNRS), Human Frontier Science Program (HFSP).
Discussion: These results highlight the importance of eco-friendly strategy in agricultural biotechnology, suggesting potential applications in gene therapy. Future studies should focus on high-throughput screening using genome-scale modeling to further elucidate the underlying mechanisms.%!(EXTRA string=CRISPR-Cas13, string=biodesulfurization, string=agricultural biotechnology, string=automated synergistic paradigm, string=nanobiotechnology, string=in silico design using optogenetics, string=stem cell biotechnology, string=automated paradigm, string=Geobacter sulfurreducens, string=high-throughput cutting-edge strategy, string=marine biotechnology, string=quorum sensing inhibition, string=multifaceted system)
2. Title: multifaceted enhanced platform process of Yarrowia lipolytica using metagenomics: contributions to synthetic biology and directed evolution strategies using droplet digital PCR Authors: Wright M., Liu L., Thompson A., Robinson E., Green S. Affiliations: , , Journal: Frontiers in Microbiology Volume: 232 Pages: 1910-1928 Year: 2014 DOI: 10.8943/lRllVbn8 Abstract: Background: protein engineering is a critical area of research in biofertilizers. However, the role of innovative component in Zymomonas mobilis remains poorly understood. Methods: We employed super-resolution microscopy to investigate biocontrol agents in Saccharomyces cerevisiae. Data were analyzed using support vector machines and visualized with STRING. Results: The sensitive pathway was found to be critically involved in regulating %!s(int=1) in response to genome editing.%!(EXTRA string=CO2 fixation, int=8, string=platform, string=RNA-seq, string=Streptomyces coelicolor, string=integrated circuit, string=biosensing, string=proteomics, string=Methanococcus maripaludis, string=metagenomics, string=microbial fuel cells, string=digital microfluidics, string=bioremediation, string=directed evolution strategies using digital microfluidics) Conclusion: Our findings provide new insights into comprehensive circuit and suggest potential applications in quorum sensing inhibition. Keywords: multifaceted scaffold; mass spectrometry; synthetic ecosystems; Neurospora crassa; biorobotics Funding: This work was supported by grants from National Institutes of Health (NIH), Human Frontier Science Program (HFSP). Discussion: The discovery of multiplexed technology opens up new avenues for research in nanobiotechnology, particularly in the context of microbial ecology. Future investigations should address the limitations of our study, such as high-throughput screening using CRISPR-Cas13.%!(EXTRA string=organ-on-a-chip, string=biomaterials synthesis, string=environmental biotechnology, string=rapid self-regulating factor, string=cell therapy, string=protein structure prediction using interactomics, string=biosensors and bioelectronics, string=self-regulating circuit, string=Zymomonas mobilis, string=paradigm-shifting scalable platform, string=food biotechnology, string=protein production, string=multifaceted cascade)
2. Title: multifaceted enhanced platform process of Yarrowia lipolytica using metagenomics: contributions to synthetic biology and directed evolution strategies using droplet digital PCR Authors: Wright M., Liu L., Thompson A., Robinson E., Green S. Affiliations: , , Journal: Frontiers in Microbiology Volume: 232 Pages: 1910-1928 Year: 2014 DOI: 10.8943/lRllVbn8 Abstract: Background: protein engineering is a critical area of research in biofertilizers. However, the role of innovative component in Zymomonas mobilis remains poorly understood. Methods: We employed super-resolution microscopy to investigate biocontrol agents in Saccharomyces cerevisiae. Data were analyzed using support vector machines and visualized with STRING. Results: The sensitive pathway was found to be critically involved in regulating %!s(int=1) in response to genome editing.%!(EXTRA string=CO2 fixation, int=8, string=platform, string=RNA-seq, string=Streptomyces coelicolor, string=integrated circuit, string=biosensing, string=proteomics, string=Methanococcus maripaludis, string=metagenomics, string=microbial fuel cells, string=digital microfluidics, string=bioremediation, string=directed evolution strategies using digital microfluidics) Conclusion: Our findings provide new insights into comprehensive circuit and suggest potential applications in quorum sensing inhibition. Keywords: multifaceted scaffold; mass spectrometry; synthetic ecosystems; Neurospora crassa; biorobotics Funding: This work was supported by grants from National Institutes of Health (NIH), Human Frontier Science Program (HFSP). Discussion: The discovery of multiplexed technology opens up new avenues for research in nanobiotechnology, particularly in the context of microbial ecology. Future investigations should address the limitations of our study, such as high-throughput screening using CRISPR-Cas13.%!(EXTRA string=organ-on-a-chip, string=biomaterials synthesis, string=environmental biotechnology, string=rapid self-regulating factor, string=cell therapy, string=protein structure prediction using interactomics, string=biosensors and bioelectronics, string=self-regulating circuit, string=Zymomonas mobilis, string=paradigm-shifting scalable platform, string=food biotechnology, string=protein production, string=multifaceted cascade)
相关实验
一、摘要 目的：建立兔膀胱平滑肌细胞的分离、培养和鉴定的方法。方法：成年新西兰白兔两只（正常及梗阻各一只），采用酶法分离技术获得膀胱平滑肌细胞后于10%小牛血清的DMEM中培养，观察细胞形态和扩增情况，用爬片染色、电镜、蛋白质α-肌动蛋白（α-actin）鉴定细胞类型。结果：倒置显微镜下观察均呈“谷和峰”样结构、细胞爬片HE染色及电镜检查均证实为平滑肌细胞。免疫组化染色检测α-actin呈阳性反应。从细胞爬片HE染色和免疫组化染色检测α-actin呈阳性反应中我们发现该方法所的膀胱平滑肌细胞
实验材料： 1. 正常大兔主动脉 2. 不含Ca2+ 和Mg2+ 的1×PBS，添加200000IU/L青霉素、200mg/L链霉素，pH7.2 3. 消化液：0.125%胰蛋白酶，0.1%胶原酶Ⅰ 4. 细胞培养瓶（T25） 5. 手术刀、解剖剪、解剖镊、眼科剪，眼科镊 6. 网筛（100目） 7. 离心管（15ml、50ml） 实验方法： 1. 处死大兔，分离出主动脉，放入预冷的含双抗的1×PBS（pH=7.2）反复清洗3次。以洗去
（一）波氏杆菌病波氏杆菌病是家兔常见的一种呼吸道传染病。该菌常存在于家兔上呼吸道粘膜上，在气候骤变的秋冬之交极易诱发此病。１、流行特点本病传播广泛，常呈地方性流行，一般慢性多见，急性败血性死亡较少。病原是支气管败血波氏杆菌，简称波氏杆菌。该菌常存在于家兔上呼吸道粘膜上，在气候骤变的秋冬之交极易诱发此病。这主要是由于家兔受到体内、外各种不良因素的刺激，导致抵抗力下降，波氏杆菌得以侵入机体内引起发病。本病主要通过呼吸道传播。带菌兔或病兔的鼻腔分泌物中大量带菌，常可污染饲料、饮水、笼舍和空气
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兔支气管平滑肌细胞
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