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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Recombinant Mouse Klrb1b Protein, N-Fc
- 供应商:
武汉益普生物科技有限公司
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1800.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2880.0 |
| 规格: | 1mg | 产品价格: | ¥17280.0 |
| 产品名 | Recombinant Mouse Klrb1b Protein, N-Fc |
| 货号 | EMN45101 |
| 别名 | Killer cell lectin-like receptor subfamily B member 1B allele A, CD161 antigen-like family member B, Lymphocyte antigen 55b, Ly-55b, NKR-P1 34, Natural killer cell surface protein NKR-P1B allele SJL/BALB, NKR-P1B, CD161b, Klrb1b, Ly55b, Nkrp1b |
| 表达系统 | Mammalian Cells |
| Accession号 | P27812 |
| 种属 | Mus musculus (Mouse) |
| 蛋白长度 | Leu64-Ser223 |
| 预测分子量 | 46.07 kDa |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |

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文献和实验【原创】Protein A Sepharose 之进化篇。。。
洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下
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Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex
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