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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Human COL17A1 Protein, N-GST & C-His
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug
| Expression system | Mammalian Cells |
| Accession | P08667 |
| Protein length | Lys20-Gly457 |
| Alternative Names | Glycoprotein, G |
| Form | Lyophilized |
| Predicted molecular weight | 56.41 kDa |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Nature | Recombi\t |
| Species | (strain Pasteur vaccins / PV) (RABV) |
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to 37°C and 65°C Sonicator Spectrophotometer Thermal cycler, automated Timer Tubes, microcentrifuge, 1.5-mL Tubes, polypropylene, 14-mL (e.g., 17- x 100-mm) for use in high-speed centrifuge Tubes, polypropylene, 50-mL
Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
. This purification process resulted in greater than 95% pure rBoNTC(Hc )-C based on SDS-PAGE, and yielded up to 0.2 g of rBoNTC(Hc )-C/kg cells. The final protein yield is a function of protein expression level during fermentation and the purification methods
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