- ￥620 - 2200
- 善本生物
- 1254
- 2025年11月15日
- Western Blotting (WB), Flow Cytometry (FCM)
- 兔
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 浓度：
Recommended Dilutions：Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
- 应用范围：
Western Blotting (WB), Flow Cytometry (FCM)
- 宿主：
兔
- 适应物种：
Human, Mouse, Rat
- 保质期：
One year
- 克隆性：
Rabbit mAb
- 保存条件：
Store at -20 °C for one year. Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
- 形态：
液体
- 亚型：
Rabbit IgG
- 免疫原：
A synthesized peptide derived from human alpha smooth muscle Actin
- 规格：
20ul/50ul/100ul
| 规格： | 20ul | 产品价格： | ￥620.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1200.0 |
| 规格： | 100ul | 产品价格： | ￥2200.0 |
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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
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