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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 保质期:
1 year
- 抗原来源:
Monkey
- 目录编号:
ZF690207
- 级别:
科研级别
- 库存:
999
- 供应商:
abinscience
- 浓度:
1mg/ml
- 抗体英文名:
Normal Monkey (Rhesus) IgG Isotype Control
- 抗体名:
Normal Monkey (Rhesus) IgG Isotype Control
- 规格:
50ug

| Product name | Normal Monkey (Rhesus) IgG Isotype Control |
|---|---|
| Catalog No. | ZF690207 |
| Host species | Monkey |
| Specificity | \ |
| Conjugation | Unconjugated |
| Species reactivity | \ |
| Immunogen | \ |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Purity | >95% as determined by SDS-PAGE. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FCM, Isotype Control |
| Target | IgG, Immune globulin G |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | \ |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Background | \ |
| Alternative Names | \ |
| Clone ID | \ |
| Species | \ |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验不同,应选用相同来源的未标记单抗作为同型对照来调整背景染色。举个例子:比如检测一抗为单抗的mouse anti rat CD11b,clone OX-42 purified IgG,那么它的isotype 是mouse IgG2a, 所以可以用purified(纯化的) mouse IgG2a来做OX-42的同型对照(Isotype Control)。一般的的生物技术公司和国内的代理都有出售。 同型对照:是指与MoAb相同的、未免疫小鼠的免疫球蛋白亚类,若使用直接
as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
结果分析的重要步骤和Trouble shooting的依据,因为非特异性的免疫沉淀难以避免。 一般的设置方法 1 未经免疫 沉淀的超声处理后样本( INPUT)。 2 加特异抗体来源动物的正常血清或无关抗体(MOCK)。 3 阳性对照引物的应用。 4 选择不与目的蛋白质结合的基因组 区域作对照。 如 阳性对照抗体 (Anti-acetyl-Histone H3) * 阴性对照抗体 (Normal Rabbit
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