- ￥1788
- abinscience
- France
- ZC273011
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in 20 mM Tris-HCl pH 8.0, 100 mM NaCl,1mM EDTA, 4%trehalose,302% mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Tupaia chinensis CD69 Protein, C-His
- 库存：
999
- 供应商：
abinscience
- 规格：
50ug
| Product name | Recombinant Tupaia chinensis CD69 Protein, C-His |
|---|---|
| Catalog No. | ZC273011 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | Mammalian cells |
| Accession | XP_006141645.1 |
| Protein length | Ser62-Gln199 |
| Nature | Recombinant |
| Predicted molecular weight | 18.52 kDa |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative Names | AIM, CD69, C-type lectin domain family 2 member C, Early T-cell activation antigen p60, BL-AC/P26, GP32/28, MLR-3, Early activation antigen CD69, Leukocyte surface antigen Leu-23, CLEC2C, EA1, Activation inducer molecule, |
| Species | Tupaia chinensis (Chinese tree shrew) |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验beads）结合细胞（刀豆蛋白 A 能与细胞膜上的糖蛋白结合）。使用非离子去污剂洋地黄皂苷进行细胞膜通透。然后分步孵育靶蛋白（如转录因子, TF）的抗体（一抗）、二抗、和 Protein A-Tn5 ，抗体和 Protein A-Tn5 能够通过细胞膜、核孔进入细胞核，Tn5 通过 Protein A 和抗体的介导切割靶蛋白附近的 DNA 序列。使用 Tn5 在进行切割的时候，会在被切割的片段两端加上接头序列，通过 PCR 扩增直接可以得到二代测序文库。 图 9: CUT&Tag、CUT
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
如下：几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利：N-端，C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合，你可以将目的基因替换MCS―Intein2―几丁质结合蛋白，如果想做C端融合表达，就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS，不用分2个载体；而且做过克隆的人会很有体会：很多时候双酶切载体，由于2个酶切位点都多克隆位点上，没有小片断切下来，所以是否成功双酶切就不好判断，如果用Twin载体就会切下一个片断
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