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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100⁺瓶
- 供应商：
上海睿安生物13611631389
- 英文名：
Histopaque®-1119
- 规格：
100ml/瓶
Sigma货号11191现货Histopaque即用型培养基13611631389上海睿安生物
说明
一般描述:Histopaque®-1119是一种无菌、经过内毒素测试的即用型培养基。该溶液由聚蔗糖和泛影酸钠组成，经调整密度为1.119 g/ml，pH为8.8–9.0。
应用:Histopaque®-1119适合与Histopaque-1077一起使用，因为它有助于从少量全血或骨髓中快速恢复活性单个核细胞和粒细胞。
特点和优势
Histopaque®-1119有许多优势，比如:
- 可使活细胞极(jí)佳恢复
- 允许选择分离不同种类的血细胞
- 防止细胞变形
- 保持细胞活力
- 最小化外来细胞干扰
- 各批次性能可重复
- 经过过滤杀菌和内毒素检测
- 在2-8°C避光保护条件下可稳定保存至少2年
原理:该过程涉及在Histopaque-1119上使等体积的Histopaque-1077分层，形成双梯度。将抗凝全血小心地铺在上层Histopaque-1077培养基上。离心后，红细胞被聚蔗糖聚集并因此迅速沉淀。粒细胞位于下部Histopaque-1077/1119界面；而在上部血浆/Histopaque-1077界面处有淋巴细胞和其它单核细胞。在上部界面中发现的大多数外部血小板在洗涤步骤中通过低速离心去除。红细胞污染可以忽略不计。
法律信息:Histopaque is a registered trademark of Merck KGaA,Darmstadt,Germany
同行评审论文(文献)
The CiaR response regulator in group B Streptococcus promotes intracellular survival and resistance to innate immune defenses.
Darin Quach et al.
Journal of bacteriology, 191(7), 2023-2032 (2008-12-31)
Group B Streptococcus (GBS) is major cause of invasive disease in newborn infants and the leading cause of neonatal meningitis. To gain access to the central nervous system (CNS), GBS must not only subvert host defenses in the bloodstream but
Nox2+ myeloid cells drive vascular inflammation and endothelial dysfunction in heart failure after myocardial infarction via angiotensin II receptor type 1.
Michael Molitor et al.
Cardiovascular research, 117(1), 162-177 (2020-02-23)
Heart failure (HF) ensuing myocardial infarction (MI) is characterized by the initiation of a systemic inflammatory response. We aimed to elucidate the impact of myelomonocytic cells and their activation by angiotensin II on vascular endothelial function in a mouse model
Identification of pneumococcal colonization determinants in the stringent response pathway facilitated by genomic diversity.
Yuan Li et al.
BMC genomics, 16, 369-369 (2015-05-10)
Understanding genetic determinants of a microbial phenotype generally involves creating and comparing isogenic strains differing at the locus of interest, but the naturally existing genomic and phenotypic diversity of microbial populations has rarely been exploited. Here we report use of
Short-Term High-Starch, Low-Protein Diet Induces Reversible Increase in β-cell Mass Independent of Body Weight Gain in Mice.
Atsushi Masuda et al.
Nutrients, 11(5) (2019-05-15)
Long-term exposure to a high starch, low-protein diet (HSTD) induces body weight gain and hyperinsulinemia concomitantly with an increase in β-cell mass (BCM) and pancreatic islets number in mice; however, the effect of short-term exposure to HSTD on BCM and
Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39.
Matthew C Hyman et al.
The Journal of clinical investigation, 119(5), 1136-1149 (2009-04-22)
Leukocyte and platelet accumulation at sites of cerebral ischemia exacerbate cerebral damage. The ectoenzyme CD39 on the plasmalemma of endothelial cells metabolizes ADP to suppress platelet accumulation in the ischemic brain. However, the role of leukocyte surface CD39 in regulating
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文献和实验该产品被引用文献
Sigma货号11191现货Histopaque即用型培养基13611631389上海睿安生物
说明
一般描述:Histopaque®-1119是一种无菌、经过内毒素测试的即用型培养基。该溶液由聚蔗糖和泛影酸钠组成，经调整密度为1.119 g/ml，pH为8.8–9.0。
应用:Histopaque®-1119适合与Histopaque-1077一起使用，因为它有助于从少量全血或骨髓中快速恢复活性单个核细胞和粒细胞。
特点和优势
Histopaque®-1119有许多优势，比如:
- 可使活细胞极(jí)佳恢复
- 允许选择分离不同种类的血细胞
- 防止细胞变形
- 保持细胞活力
- 最小化外来细胞干扰
- 各批次性能可重复
- 经过过滤杀菌和内毒素检测
- 在2-8°C避光保护条件下可稳定保存至少2年
原理:该过程涉及在Histopaque-1119上使等体积的Histopaque-1077分层，形成双梯度。将抗凝全血小心地铺在上层Histopaque-1077培养基上。离心后，红细胞被聚蔗糖聚集并因此迅速沉淀。粒细胞位于下部Histopaque-1077/1119界面；而在上部血浆/Histopaque-1077界面处有淋巴细胞和其它单核细胞。在上部界面中发现的大多数外部血小板在洗涤步骤中通过低速离心去除。红细胞污染可以忽略不计。
法律信息:Histopaque is a registered trademark of Merck KGaA,Darmstadt,Germany
同行评审论文(文献)
The CiaR response regulator in group B Streptococcus promotes intracellular survival and resistance to innate immune defenses.
Darin Quach et al.
Journal of bacteriology, 191(7), 2023-2032 (2008-12-31)
Group B Streptococcus (GBS) is major cause of invasive disease in newborn infants and the leading cause of neonatal meningitis. To gain access to the central nervous system (CNS), GBS must not only subvert host defenses in the bloodstream but
Nox2+ myeloid cells drive vascular inflammation and endothelial dysfunction in heart failure after myocardial infarction via angiotensin II receptor type 1.
Michael Molitor et al.
Cardiovascular research, 117(1), 162-177 (2020-02-23)
Heart failure (HF) ensuing myocardial infarction (MI) is characterized by the initiation of a systemic inflammatory response. We aimed to elucidate the impact of myelomonocytic cells and their activation by angiotensin II on vascular endothelial function in a mouse model
Identification of pneumococcal colonization determinants in the stringent response pathway facilitated by genomic diversity.
Yuan Li et al.
BMC genomics, 16, 369-369 (2015-05-10)
Understanding genetic determinants of a microbial phenotype generally involves creating and comparing isogenic strains differing at the locus of interest, but the naturally existing genomic and phenotypic diversity of microbial populations has rarely been exploited. Here we report use of
Short-Term High-Starch, Low-Protein Diet Induces Reversible Increase in β-cell Mass Independent of Body Weight Gain in Mice.
Atsushi Masuda et al.
Nutrients, 11(5) (2019-05-15)
Long-term exposure to a high starch, low-protein diet (HSTD) induces body weight gain and hyperinsulinemia concomitantly with an increase in β-cell mass (BCM) and pancreatic islets number in mice; however, the effect of short-term exposure to HSTD on BCM and
Self-regulation of inflammatory cell trafficking in mice by the leukocyte surface apyrase CD39.
Matthew C Hyman et al.
The Journal of clinical investigation, 119(5), 1136-1149 (2009-04-22)
Leukocyte and platelet accumulation at sites of cerebral ischemia exacerbate cerebral damage. The ectoenzyme CD39 on the plasmalemma of endothelial cells metabolizes ADP to suppress platelet accumulation in the ischemic brain. However, the role of leukocyte surface CD39 in regulating
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Sigma货号11191现货Histopaque即用型培养基13611631389上海睿安生物
￥870.48
