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T7 RNA polymerase（T7 RNA聚合酶）是一种高度特异识别T7启动子序列的DNA依赖的5'→3' RNA聚合酶
- 体外mRNA合成：T7 RNA polymerase用于体外mRNA合成，具有高度特异性，专门识别T7噬菌体启动子。
- 标记RNA合成：该酶可以识别修饰的NTP（如生物素标记、荧光素标记的NTP），用于合成各种标记的RNA。
- RNA转录：T7 RNA polymerase以含有T7启动子序列的单链或双链DNA为模板，NTP为底物，合成与启动子下游的单链DNA或双链DNA模板链互补的RNA
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文献和实验mRNA Amplification with T7 RNA Polymerase
on a 42ºC oven and set PCR machine to hold at 70ºC. In an Eppendorf tube add: 1 µg Total RNA 1 µL T7 oligo(dT) primer q.s. to 12 µL with Nuclease-free H2 O Incubate 10 min. at 70ºC. Spin briefly to pull
Oocyte Expression With Injection of Purified T7 RNA Polymerase
of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
Ultraviolet (UV)-dependent photochemical crosslinking is a powerful approach that can be used for the identification of RNA-protein interactions. Although 8-azidoATP (8-N3 ATP) has been widely used to elucidate the ATP binding site
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