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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Rabbit IgG
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Biotinylated
- 适应物种:
Human
- 保质期:
12个月
- 级别:
仅用于科研
- 库存:
2-3 weeks
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Rabbit
- 靶点:
HER3
- 抗体英文名:
Biotinylated Anti-Her3 antibody(DM175); Rabbit mAb
- 抗体名:
Biotinylated Anti-Her3 antibody(DM175); Rabbit mAb
- 规格:
50μg
| SKU | DME100175B |
|---|---|
| 产品分类 | Monoclonal Antibodies |
| 规格 | Price: 10μg ¥1030.00 ; 50μg ¥3380.00 ; 100 μg ¥6080.00 ; 500 μg ¥19880.00 |
| 背景 | This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However; it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and:or overexpression of its protein have been reported in numerous cancers; including prostate; bladder; and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported; but they have not been thoroughly characterized. |
| Clone ID | DM175 |
| 别名 | HER3; ERBB3 |
| 应用 | ELISA; Flow Cyt |
| 形态与组成成分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 纯化方式 | Purified from cell culture supernatant by affinity chromatography |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 靶点 | HER3 |
| Uniprot ID | P21860 |
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文献和实验祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
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,EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体,而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体:hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本:蜡包埋人类肺部肿瘤抗原修复缓冲液:柠檬酸盐缓冲液(左)或者 EDTA 缓冲液(右)煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后,才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然,一些研究人员也使用水浴锅,但是,因为该步骤中所选择的装置对染色存在积极或消极的影响
: Indirect immunohistochemistry with avidin-biotin peroxidase(e.g rabbit anti antigen X, goat anti-rabbit biotin, avidin HRP) 1- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 has been added. 2- Briefly blot the slides without letting
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