- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Agrobacterium tumefaciens KYC 55
- 保质期:
3年
- 供应商:
百欧博伟
- 保存条件:
-80
平台编号:bio-104365
Bioassay protocol
1. 菌株
Agrobacterium tumefaciens KYC55(pJZ372)(pJZ384)(pJZ410) ,AHL高效检测菌株,Tc2 uM/mL, Spe100, Gm100,28℃
2. 保存Preinduced cell(简称pre)
2.1 接种KYC55至LB中活化,加入相应抗生素。 Tc2 uM/mL, Spe100, Gm100,28℃ 四环素2 uM/mL, 壮观100uM/mL, 庆大霉素100uM/mL
2.2 长出后,1%转接至AT medium中,28℃培养,长至OD600约0.6左右。
2.3 10000rpm×10min离心,去除上清。以“AT/甘油=20”的量加入20%甘油(即浓缩20倍),分装至1.5ml离心管中,-20℃保存。
3. 制备待检测菌上清
3.1 培养待测菌至OD600 1.5左右,12000rpm×2min离心保存上清。多余上清或暂时来不及做assay的上清可以保存在-20℃。
参考文献:zhu2003
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Electroporation of Agrobacterium tumefaciens
Agrobacterium tumefaciens is a soil bacterium that causes tumors on dicotyledonous plants. Virulent strains harbor a large plasmid, the Ti (tumor-inducing) plasmid, which is involved in tumorigenesis. A small segment of this plasmid, the T
Agrobacterium tumefaciens Chemotaxis Protocols
Directed movement in response to chemical attractants is of crucial importance to Agrobacterium tumefaciens . Motility is a determinant of rhizosphere competence (1 ), and chemotaxis a conditional requirement for virulence
Root Transformation by Agrobacterium tumefaciens
and molecular analysis of higher plants have produced methods for in vitro propagation (1 ), regeneration and transformation (2 )–(15 ) using either Agrobacterium or direct gene transfer.
技术资料暂无技术资料 索取技术资料









