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- 2026年04月13日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
联科生物
- 规格：
10μg/50μg/100μg
| 规格： | 10μg | 产品价格： | ￥700.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2500.0 |
| 规格： | 100μg | 产品价格： | ￥3300.0 |
General Information
| SOURCE |
Escherichia coli |
|---|---|
| SYNONYMS |
Leukemia inhibitory factor; CDF; DIA; HILDA |
| ACCESSION |
P09056 |
| MOLECULAR MASS |
19.9 KDa |
| DESCRIPTION |
Recombinant Mouse LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds. |
| SHIPPING |
The product is shipped at 4 ℃ . Upon receipt, store it immediately at the temperature recommended below. |
| STABILITY & STORAGE |
Aliquot the reconstituted solution to minimize freeze-thaw cycles. Lyophilized protein should be stored at -20℃ to -80℃, stable for one year after receipt. Aliquots of reconstituted samples are stable at ≤ -20℃ for 3 months. |
| RECONSTITUTION |
Centrifuge tubes before opening. Dissolve the lyophilized protein in distilled water. Do not mix by vortex. It is recommended to dissolve the product at a concentration of twice the specified specification. |
| BACKGROUND |
Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNF-α and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal. |
Specifications
| FORMULATION |
Lyophilized from sterile PBS, pH 7.4, 5% trehalose. |
|---|---|
| PURITY |
≥95% as determined by SDS-PAGE |
| ENDOTOXIN |
< 1EU/ug |
| BIO ACTIVITY |
Immobilized Anti-Mouse LIF Antibody at 0.2 ug/mL (50 uL/well) can bind Mouse LIF with a linear range of 48.83 - 6,250 pg/mL. |
Data
Greater than 95% as determined by reducing SDS-PAGE.
Immobilized Anti-Mouse LIF Antibody at 0.2 ug/mL (50 uL/well) can bind Mouse LIF with a linear range of 48.83 - 6,250 pg/mL. (EC50 = 0.76 ng/mL)
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文献和实验with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
Isolation and Culture of Mouse Germ Cells
cells have subsequently been found to be physiologically relevant to germ cell growth and development in vivo. For example, leukemia inhibitory factor (LIF) was identified as a potent germ cell survival factor and mitogen in culture (1 ). Subsequently
Purification of Recombinant Poly(ADP-Ribose) Polymerases
, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.
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