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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Canine IgG3, lambda Isotype Control Antibody (HyHEL-10), PerCP
- 抗体英文名:
Canine IgG3, lambda Isotype Control Antibody (HyHEL-10), PerCP
- 浓度:
0.5 mg/ml
- 应用范围:
FCM, Isotype Control
- 宿主:
Dog
- 保质期:
1 year
- 级别:
科研级
- 库存:
999
- 供应商:
AntibodySystem
- 克隆性:
Monoclonal
- 保存条件:
Store at 4°C for 12 months. Protect from light. Do not freeze.
- 亚型:
IgG3, lambda
- 规格:
100T

| 宿主 | Dog |
| 偶联物 | PerCP |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| 浓度 | 0.5 mg/ml |
| 克隆类型 | Monoclonal |
| 同种型 | IgG3, lambda |
| 应用 | FCM, Isotype Control |
| 内毒素水平 | Please contact with the lab for this information. |
| 稳定性和存储 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 克隆号 | HyHEL-10 |
| 备注 | For research use only. |
Our products are designed for various research areas, supporting studies on 48 types of viruses, superbugs, parasites, cancer, Alzheimer's disease, Parkinson's disease, allergic responses, immune suppression, and immune activation.
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
as many as 6 million dpm to maximize sensitivity. 4) Put the 100,000 dpm samples aside until step #12. 5) After lyophilization, solubilize 200,000 dpm samples in 350 µl IMP buffer. 6) Add 50 µl of antibody or control (normal rabbit serum, mouse isotype control
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
of antibody or control (normal rabbit serum, mouse isotype control,, etc.) to each tube and incubate overnight for 6 h at room temperature. 7) Add 100 µl of Protein-A-Sepharose solution (shake to suspend slurry before pipetting) and incubate on a tube
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