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- AntibodySystem
- FHD72213
- France
- 2025年07月12日
- FCM
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-Human CD27 Antibody (1F5), APC
- 抗体英文名：
Anti-Human CD27 Antibody (1F5), APC
- 浓度：
0.5 mg/ml
- 应用范围：
FCM
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
1 year
- 级别：
科研级
- 库存：
999
- 供应商：
AntibodySystem
- 克隆性：
Monoclonal
- 保存条件：
Store at 4°C for 12 months. Protect from light. Do not freeze.
- 亚型：
IgG1
- 规格：
100T
| 宿主 | Mouse |
| 偶联物 | APC |
| 种属反应性 | Human |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| 浓度 | 0.5 mg/ml |
| 克隆类型 | Monoclonal |
| 同种型 | IgG1 |
| 应用 | FCM |
| 靶标 | CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27 |
| 内毒素水平 | Please contact with the lab for this information. |
| 稳定性和存储 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 克隆号 | 1F5 |
| 备注 | For research use only. |
AntibodySystem's product range supports research in multiple fields, encompassing 48 types of viruses, superbugs, parasites, oncology, Alzheimer's, Parkinson's, hypersensitivity, immune suppression, and immune activation.
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文献和实验(1 ). In our laboratory, we have developed methods to immortalize specific antibody-producing cells by fusing secreting EBV-activated lymphocytes to mouse-human heteromyeloma cell lines with electrofusion, followed by cloning (2 ). This methodology has allowed
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
, and has characterized pathways for the trafficking of APC both into and out of the nucleus.Antibody specificity is an important factor in the determination of APC localization, and in this chapterwe outline a strategy for the unambiguous detection of APC using
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
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