Olaratumab ELISA Kit ELISA试剂盒

Olaratumab ELISA Kit ELISA试剂盒

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  • ¥6768
  • AntibodySystem已认证
  • 156.25 - 10,000 ng/mL
  • 112.17 ng/mL
  • KDD16101
  • France
  • 2025年07月16日
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    • 详细信息
    • 技术资料
    • 库存

      999

    • 供应商

      AntibodySystem

    • 检测范围

      156.25 - 10,000 ng/mL

    • 检测方法

      Colorimetric

    • 应用

      Used for the quantitative determination of Olaratumab concentration in serum and plasma.

    • 标记物

      HRP

    • 样本

      Plasma, Serum

    • 灵敏度

      112.17 ng/mL

    • 规格

      96T

    Olaratumab ELISA Kit ELISA试剂盒

    描述PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human CD140a has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Olaratumab in the sample competitively binds to the pre-coated protein with biotin-labeled Olaratumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Olaratumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
    应用Used for the quantitative determination of Olaratumab concentration in serum and plasma.
    稳定性和存储When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
    检测方法Colorimetric
    精准度

    Intra-Assay Precision (Precision within an assay): <20%

    Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

    Inter-Assay Precision (Precision between assays): <20%

    Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    16

    16

    16

    24

    24

    24

    Mean (ng/mL)

    5830.6

    1331.4

    305.7

    6891.0

    1451.8

    310.8

    Standard deviation

    478.4

    117.8

    41.8

    1025.4

    194.1

    58.7

    CV (%)

    8.2

    8.8

    13.7

    14.9

    13.4

    18.9

     

    样本类型Plasma, Serum
    实验类型Quantitative
    灵敏度112.17 ng/mL
    检测范围156.25 - 10,000 ng/mL
    回收率80-120%
    背景Olaratumab (IMC-3G3) is a fully human IgG1 monoclonal antibody developed by Eli Lilly and Company. It selectively binds the external domain of human platelet-derived growth factor receptor (PDGFR). With the trade name Lartruvo, olaratumab has been approved by USA, Canada, and European Union to use in combination with doxorubicin for the treatment of adults with advanced soft-tissue sarcoma (STS) who cannot be cured by cancer surgery or radiation therapy, and who have not been previously treated with doxorubicin.
    别名3G3, IMC-3G3, LY3012207, CAS: 1024603-93-7
    运输2-8 ℃
    备注For Research Use Only.

    Established in 2019 in France, AntibodySystem Laboratories SAS is a standalone brand dedicated to the production of protein and antibody reagents for life science research. We aim to empower researchers globally with high-quality products for fundamental scientific research. AntibodySystem offers a broad selection, including traditional polyclonal antibodies, essential housekeeping antibodies, tag antibodies, high-potency proteins, functional antibodies for in vivo use, low-background flow antibodies, highly specific nanobodies, unique small molecule antibodies, high-quality phospho-specific antibodies, DNA/RNA antibodies, and PEG antibodies, among others.
    AntibodySystem's product range supports research in multiple fields, encompassing 48 types of viruses, superbugs, parasites, oncology, Alzheimer's, Parkinson's, hypersensitivity, immune suppression, and immune activation.

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