HPAFII细胞

HPAF-II/HPAF-II细胞系/HPAF-II细胞株/HPAF-II人胰腺癌细胞

Cell line name HPAF-II

Synonyms HPAF II; HPAFII; HPAF-2; HPAF2; HPAF/CD18; CD18/HPAF; HPAF-II/CD18; CD-18; CD18; CD 18

Accession CVCL_0313

Resource Identification Initiative To cite this cell line use: HPAF-II (RRID:CVCL_0313)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: KuDOS 95 cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: NCI RAS program mutant KRAS cell line panel.

Population: Caucasian.

Doubling time: 26 hours (PubMed=2734279); 70 hours (PubMed=25984343).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: CRISPR phenotypic screen.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: DNA methylation analysis.

Omics: Metabolome analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: Proteome analysis by 2D-DE/MS.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Caution: Could not find proof that the CD18 subline of HPAF is really HPAF-II but many indirect evidences points in this direction. If someone read this comment and can help us, please do.

Caution: TP53 mutation indicated incorrectly as being at c.452C>T in PubMed=1764370.

Misspelling: HPAF11; PubMed=25984343.

Misspelling: Hapaf-II; Note=Occasionally.

Derived from site: Metastatic; Ascites; UBERON=UBERON_0007795.

PubMed=1764370; DOI=10.1038/bjc.1991.467; PMCID=PMC1977874

Barton C.M., Staddon S.L., Hughes C.M., Hall P.A., O'Sullivan C., Kloppel G., Theis B., Russell R.C.G., Neoptolemos J., Williamson R.C.N., Lane D.P., Lemoine N.R.

Abnormalities of the p53 tumour suppressor gene in human pancreatic cancer.

Br. J. Cancer 64:1076-1082(1991)

 

PubMed=11787853; DOI=10.1007/s004280100474

Moore P.S., Sipos B., Orlandini S., Sorio C., Real F.X., Lemoine N.R., Gress T.M., Bassi C., Kloppel G., Kalthoff H., Ungefroren H., Lohr J.-M., Scarpa A.

Genetic profile of 22 pancreatic carcinoma cell lines. Analysis of K-ras, p53, p16 and DPC4/Smad4.

Virchows Arch. 439:798-802(2001)

 

PubMed=12068308; DOI=10.1038/nature00766

Davies H.R., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.

Mutations of the BRAF gene in human cancer.

Nature 417:949-954(2002)

 

PubMed=12692724; DOI=10.1007/s00428-003-0784-4

Sipos B., Moser S., Kalthoff H., Torok V., Lohr J.-M., Kloppel G.

A comprehensive characterization of pancreatic ductal carcinoma cell lines: towards the establishment of an in vitro research platform.

Virchows Arch. 442:444-452(2003)

 

PubMed=15126341; DOI=10.1158/0008-5472.CAN-03-3159

Heidenblad M., Schoenmakers E.F.P.M., Jonson T., Gorunova L., Veltman J.A., van Kessel A.G., Hoglund M.

Genome-wide array-based comparative genomic hybridization reveals multiple amplification targets and novel homozygous deletions in pancreatic carcinoma cell lines.

Cancer Res. 64:3052-3059(2004)

 

PubMed=15367885; DOI=10.1097/00006676-200410000-00004

Loukopoulos P., Kanetaka K., Takamura M., Shibata T., Sakamoto M., Hirohashi S.

Orthotopic transplantation models of pancreatic adenocarcinoma derived from cell lines and primary tumors and displaying varying metastatic activity.

Pancreas 29:193-203(2004)

 

PubMed=15367897; DOI=10.1097/00006676-200410000-00016

Rajasekaran S.A., Gopal J., Espineda C., Ryazantsev S., Schneeberger E.E., Rajasekaran A.K.

HPAF-II, a cell culture model to study pancreatic epithelial cell structure and function.

Pancreas 29:e77-e83(2004)