MRC-5人胚肺成纤维细胞

MRC-5/MRC-5细胞系/MRC-5细胞株/MRC-5人胚肺成纤维细胞

Cell line name MRC-5

Synonyms MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5

Accession CVCL_0440

Resource Identification Initiative To cite this cell line use: MRC-5 (RRID:CVCL_0440)

Comments Group: Vaccine production cell line.

Biotechnology: Used for the production of the Human Diploid Cell Vaccine (HDCV) against rabies (Trade name: Imovax Rabies).

Biotechnology: Used for the production of the Hepatitis A vaccines Avaxim, Epaxal, Havrix and Vaqta.

Biotechnology: Used for the production of the live varicella virus vaccine (Trade name: Varivax).

Biotechnology: Used for the production of the live zoster vaccine (Trade name: Zostavax).

Senescence: Capable of attaining 42-46 population doublings before the onset of senescence (ATCC=CCL-171). Senesces at 26 PDL (PubMed=17395773).

Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).

Doubling time: 45 hours (Note=Lot 08072009), ~40 hours (Note=Lot 02272015) (JCRB=IFO50073); 36 hours (Note=Lot 041392), ~5 days (Note=Lot 031098), ~2 days (Note=Lot 05192003), ~1.5 days (Note=Lot 05202005), ~34 hours (Note=Lot 06022011), ~38 hours (Note=Lot 04212016) (JCRB=JCRB9008).

Omics: DNA methylation analysis.

Omics: Glycosphingolipids analysis.

Omics: Proteome analysis by 2D-DE.

Omics: Proteome analysis by 2D-DE/MS.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Misspelling: MCR-5; Note=Occasionally.

Derived from site: In situ; Fetal lung; UBERON=UBERON_0005597.

Cell type: Fibroblast of lung; CL=CL_0002553.

PubMed=3335022

Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Cancer Res. 48:589-601(1988)

 

PubMed=1965304; DOI=10.1002/elps.1150111203

Celis J.E., Dejgaard K., Madsen P., Leffers H., Gesser B., Honore B., Rasmussen H.H., Olsen E., Lauridsen J.B., Ratz G., Mouritzen S., Basse B., Hellerup M., Celis A., Puype M., Van Damme J., Vandekerckhove J.

The MRC-5 human embryonal lung fibroblast two-dimensional gel cellular protein database: quantitative identification of polypeptides whose relative abundance differs between quiescent, proliferating and SV40 transformed cells.

Electrophoresis 11:1072-1113(1990)

 

PubMed=10752125

Poljanskaia G.G., Efremova T.N., Sakuta G.A.

The effect of mycoplasmal contamination of human embryonic lung cell line MRC-5 on the karyotypic variability.

Tsitologiia 42:190-195(2000)

 

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459

Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.

Short tandem repeat profiling provides an international reference standard for human cell lines.

Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

 

PubMed=17395773; DOI=10.1634/stemcells.2006-0504

Sudo K., Kanno M., Miharada K., Ogawa S., Hiroyama T., Saijo K., Nakamura Y.

Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.

Stem Cells 25:1610-1617(2007)

 

PubMed=19657000

Rubporn A., Srisomsap C., Subhasitanont P., Chokchaichamnankit D., Chiablaem K., Svasti J., Sangvanich P.

Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line.

Cancer Genomics Proteomics 6:229-237(2009)

 

PubMed=21637780; DOI=10.1371/journal.pgen.1002085; PMCID=PMC3102737

Nishino K., Toyoda M., Yamazaki-Inoue M., Fukawatase Y., Chikazawa E., Sakaguchi H., Akutsu H., Umezawa A.

DNA methylation dynamics in human induced pluripotent stem cells over time.

PLoS Genet. 7:E1002085-E1002085(2011)

 

PubMed=25877200; DOI=10.1038/nature14397

Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.

A resource for cell line authentication, annotation and quality control.

Nature 520:307-311(2015)

 

PubMed=25903999; DOI=10.1002/biot.201400388

Genzel Y.

Designing cell lines for viral vaccine production: where do we stand?

Biotechnol. J. 10:728-740(2015)

 

PubMed=26477663; DOI=10.1038/srep14988; PMCID=PMC4609952

Ojima T., Shibata E., Saito S., Toyoda M., Nakajima H., Yamazaki-Inoue M., Miyagawa Y., Kiyokawa N., Fujimoto J.-i., Sato T., Umezawa A.

Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells.

Sci. Rep. 5:14988-14988(2015)

 

CLPUB00391

Wadman M.

The vaccine race: science; politics; and the human costs of defeating disease.

(In book) ISBN 9780525427537; pp.1-436; Viking; New York; USA (2016)

 

PubMed=31869419; DOI=10.1084/jem.20191164; PMCID=PMC7062530

Lubke M., Spalt S., Kowalewski D.J., Zimmermann C., Bauersfeld L., Nelde A., Bichmann L., Marcu A., Peper J.K., Kohlbacher O., Walz J.S., Le-Trilling V.T.K., Hengel H., Rammensee H.-G., Stevanovic S., Halenius A.

Identification of HCMV-derived T cell epitopes in seropositive individuals through viral deletion models.

J. Exp. Med. 217:e20191164.1-e20191164.14(2020)

 

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494

Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.

Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.

Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)