OP9

OP9/OP9细胞系/OP9细胞株/OP9小鼠骨髓基质细胞

Cell line name    OP9
Synonyms    OP-9
Accession    CVCL_4398
Resource Identification Initiative    To cite this cell line use: OP9 (RRID:CVCL_4398)
Comments    Characteristics: Does not produce Csf1 (M-CSF) due to a mutation in the gene.
Characteristics: Used as feeder cells for the differentiation of ES cells and iPS cells into hematopoietic cells.
Doubling time: ~26 hours (ATCC=CRL-2749).
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Cell type: Mesenchymal stem cell of bone marrow; CL=CL_0002540.
Species of origin    Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: (C57BL/6 x C3H) F2-op/op.
Hierarchy    Children:
CVCL_HC57 (OP-9S)    CVCL_KB57 (OP9-K)    CVCL_B217 (OP9/G)
CVCL_B218 (OP9/G-DLL1)    CVCL_B219 (OP9/N)
Sex of cell    Sex unspecified
Age at sampling    Embryo
Category    Stromal cell line
PubMed=8066449; DOI=10.1126/science.8066449
Nakano T., Kodama H., Honjo T.
Generation of lymphohematopoietic cells from embryonic stem cells in culture.
Science 265:1098-1101(1994)

PubMed=8990620; DOI=10.1016/s0925-5710(96)00531-2
Nakano T.
In vitro development of hematopoietic system from mouse embryonic stem cells: a new approach for embryonic hematopoiesis.
Int. J. Hematol. 65:1-8(1996)

PubMed=20659712; DOI=10.1016/S1673-8527(09)60067-9
Gao J., Yan X.-L., Li R., Liu Y., He W.-Y., Sun S.-K., Zhang Y., Liu B., Xiong J.-X., Mao N.
Characterization of OP9 as authentic mesenchymal stem cell line.
J. Genet. Genomics 37:475-482(2010)

PubMed=20810995; DOI=10.4049/jimmunol.1000195
Murata A., Okuyama K., Sakano S., Kajiki M., Hirata T., Yagita H., Zuniga-Pflucker J.C., Miyake K., Akashi-Takamura S., Moriwaki S., Niida S., Yoshino M., Hayashi S.-i.
A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells.
J. Immunol. 185:3905-3912(2010)

PubMed=23469264; DOI=10.1371/journal.pone.0058123; PMCID=PMC3585802
Matsubara Y., Ono Y., Suzuki H., Arai F., Suda T., Murata M., Ikeda Y.
OP9 bone marrow stroma cells differentiate into megakaryocytes and platelets.
PLoS ONE 8:E58123-E58123(2013)

PubMed=28176856; DOI=10.1038/srep42104; PMCID=PMC5296734
Kassotis C.D., Masse L., Kim S., Schlezinger J.J., Webster T.F., Stapleton H.M.
Characterization of adipogenic chemicals in three different cell culture systems: implications for reproducibility based on cell source and handling.
Sci. Rep. 7:42104-42104(2017)