HUH-1

Huh-1/Huh-1细胞系/Huh-1细胞株/Huh-1人肝癌细胞

Cell line name HuH-1

Synonyms huH-1; HUH-1; huH 1; HuH1; HUh1; HUH1; huH1

Accession CVCL_2956

Resource Identification Initiative To cite this cell line use: HuH-1 (RRID:CVCL_2956)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: Liver Cancer Model Repository (LIMORE).

Part of: MD Anderson Cell Lines Project.

Part of: TCGA-110-CL cell line panel.

Population: Japanese.

Virology: Contains multiple copies of integrated hepatitis B virus (HBV) genomes.

Doubling time: 28.23 hours (PubMed=31378681); ~36 hours (Note=Lot 02172003), ~31 hours (Note=Lot 07192012), ~39 hours (Note=Lot 08102017), ~35 hours (Note=Lot 04022021) (JCRB=JCRB0199).

Microsatellite instability: Stable (MSS) (Sanger).

Transformant: NCBI_TaxID; 10407; Hepatitis B virus (HBV).

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Genome sequenced.

Omics: miRNA expression profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Liver; UBERON=UBERON_0002107.

Sequence variations

Mutation; HGNC; 903; AXIN1; Simple; p.Asn302Profs*111 (c.904_907delAACC); Zygosity=Unspecified (PubMed=31395879).

Mutation; HGNC; 11998; TP53; Simple; p.Arg110Leu (c.329G>T); ClinVar=VCV000406597; Zygosity=Homozygous (PubMed=31395879; Cosmic-CLP=1298146; DepMap=ACH-000475).

HLA typing Source: PubMed=26589293

Class I

HLA-A A*02:06,11:01

HLA-B B*08:20,35:08

HLA-C C*01:03,07:02

Class II

HLA-DR DRB1*04:05,13:03

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0.02

Native American 0.87

East Asian, North 80.09

East Asian, South 18.1

South Asian 0

European, North 0

European, South 0.92

Disease Adult hepatocellular carcinoma (NCIt: C7956)

Adult hepatocellular carcinoma (ORDO: Orphanet_210159)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Sex of cell Male

Age at sampling 53Y

Category Cancer cell line

STR profile Source(s): Cosmic-CLP=1298146; JCRB=JCRB0199; PubMed=25877200; PubMed=31378681

 

Markers:

Amelogenin X,Y

CSF1PO 11

D3S1358 16,17

D5S818 10,13

D7S820 11

D8S1179 10,12

D13S317 10,12

D16S539 9

D18S51 16

D21S11 33.2

FGA 24

Penta D 12

Penta E 16,18

TH01 6,9

TPOX 8,11

vWA 17,18

 

Run an STR similarity search on this cell line

Publications

PubMed=6268479

Huh N.-H., Utakoji T.

Production of HBs-antigen by two new human hepatoma cell lines and its enhancement by dexamethasone.

Gann 72:178-179(1981)

 

PubMed=6310497; DOI=10.1093/nar/11.16.5391; PMCID=PMC326285

Koike K., Kobayashi M., Mizusawa H., Yoshida E., Yaginuma K., Taira M.

Rearrangement of the surface antigen gene of hepatitis B virus integrated in the human hepatoma cell lines.

Nucleic Acids Res. 11:5391-5402(1983)

 

DOI=10.1007/978-4-431-68349-0_4

Alexander J.J.

Human hepatoma cell lines.

(In book chapter) Neoplasms of the liver; Okuda K., Ishak K.G. (eds.); pp.47-56; Springer; Tokyo; Japan (1987)

 

PubMed=8389256; DOI=10.1093/carcin/14.5.987

Hsu I.C., Tokiwa T., Bennett W., Metcalf R.A., Welsh J.A., Sun T., Harris C.C.

p53 gene mutation and integrated hepatitis B viral DNA sequences in human liver cancer cell lines.

Carcinogenesis 14:987-992(1993)

 

PubMed=8835345; DOI=10.1002/(SICI)1096-9071(199602)48:2<133::AID-JMV3>3.0.CO;2-A

Tsuboi S., Nagamori S., Miyazaki M., Mihara K., Fukaya K.-i., Teruya K., Kosaka T., Tsuji T., Namba M.

Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.

J. Med. Virol. 48:133-140(1996)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

 

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027

Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Nature 483:603-607(2012)

 

PubMed=23505090; DOI=10.1002/hep.26402

Wang K., Lim H.Y., Shi S., Lee J., Deng S.-B., Xie T., Zhu Z., Wang Y.-L., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.-C.

Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.

Hepatology 58:706-717(2013)

 

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)