MG-63

MG-63/MG-63细胞系/MG-63细胞株/MG-63人骨肉瘤细胞

Cell line name MG-63

Synonyms M-G63; MG63

Accession CVCL_0426

Resource Identification Initiative To cite this cell line use: MG-63 (RRID:CVCL_0426)

Comments Group: Space-flown cell line (cellonaut).

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Population: Caucasian.

Characteristics: Can be induced to differentiate by treating the cells with 1,25-dihydroxyvitamin D3 and TGF-beta. The cells do not differentiate into the mineralized state, but stop at the matrix maturation stage (PubMed=1577731).

Doubling time: 38 hours (PubMed=218153); 28.3 hours (PubMed=19363654); ~48 hours (PubMed=21519327).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: H3K4me1 ChIP-seq epigenome analysis.

Omics: H3K27ac ChIP-seq epigenome analysis.

Omics: Metabolome analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Anecdotal: Have been flown in space on Foton-10 and Foton-M3 to study if differentiation is altered in microgravity (PubMed=9144345; PubMed=9600771; PubMed=24903274).

Caution: The reported STR profile from CLS of this cell line was changed in June 2019. Seven conflicts with other sources were resolved.

Misspelling: MGG3; PubMed=2823272; Note=In figure 4.

Derived from site: Metastatic; Bone, left femur; UBERON=UBERON_0000981.

PubMed=218153; DOI=10.1159/000225298

Heremans H., Billiau A., Cassiman J.-J., Mulier J.C., De Somer P.

In vitro cultivation of human tumor tissues. II. Morphological and virological characterization of three cell lines.

Oncology 35:246-252(1978)

 

PubMed=2823272; DOI=10.1073/pnas.84.21.7716; PMCID=PMC299371

Masuda H., Miller C., Koeffler H.P., Battifora H.A., Cline M.J.

Rearrangement of the p53 gene in human osteogenic sarcomas.

Proc. Natl. Acad. Sci. U.S.A. 84:7716-7719(1987)

 

PubMed=2233717; DOI=10.1128/mcb.10.11.5772-5781.1990; PMCID=PMC361354

Diller L., Kassel J., Nelson C.E., Gryka M.A., Litwak G., Gebhardt M., Bressac B., Ozturk M., Baker S.J., Vogelstein B., Friend S.H.

p53 functions as a cell cycle control protein in osteosarcomas.

Mol. Cell. Biol. 10:5772-5781(1990)

 

PubMed=1577731; DOI=10.1016/S0021-9258(19)50371-4

Bonewald L.F., Kester M.B., Schwartz Z., Swain L.D., Khare A., Johnson T.L., Leach R.J., Boyan B.D.

Effects of combining transforming growth factor beta and 1,25-dihydroxyvitamin D3 on differentiation of a human osteosarcoma (MG-63).

J. Biol. Chem. 267:8943-8949(1992)

 

PubMed=7873286; DOI=10.1016/8756-3282(94)90305-0

Clover J., Gowen M.

Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype?

Bone 15:585-591(1994)

 

PubMed=8617485; DOI=10.1016/S0046-8177(96)90115-X

Scotlandi K., Serra M., Manara M.C., Benini S., Sarti M., Maurici D., Lollini P.-L., Picci P., Bertoni F., Baldini N.

Immunostaining of the p30/32MIC2 antigen and molecular detection of EWS rearrangements for the diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumor.

Hum. Pathol. 27:408-416(1996)

 

PubMed=9144345; DOI=10.1359/jbmr.1997.12.5.786

Carmeliet G., Nys G., Bouillon R.

Microgravity reduces the differentiation of human osteoblastic MG-63 cells.

J. Bone Miner. Res. 12:786-794(1997)

 

PubMed=9600771; DOI=10.1016/S8756-3282(98)00007-6

Carmeliet G., Nys G., Stockmans I., Bouillon R.

Gene expression related to the differentiation of osteoblastic cells is altered by microgravity.

Bone 22:139S-143S(1998)

 

PubMed=12645653; DOI=10.1016/S0165-4608(02)00685-4

Ozaki T., Neumann T., Wai D.H., Schaefer K.-L., van Valen F., Lindner N., Scheel C., Boecker W., Winkelmann W., Dockhorn-Dworniczak B., Horst J., Poremba C.

Chromosomal alterations in osteosarcoma cell lines revealed by comparative genomic hybridization and multicolor karyotyping.

Cancer Genet. Cytogenet. 140:145-152(2003)

 

PubMed=15736406

Pautke C., Schieker M., Tischer T., Kolk A., Neth P., Mutschler W., Milz S.

Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts.

Anticancer Res. 24:3743-3748(2004)

 

PubMed=17981215; DOI=10.1016/j.cancergencyto.2007.08.003

Selvarajah S., Yoshimoto M., Maire G., Paderova J., Bayani J., Squire J.A., Zielenska M.

Identification of cryptic microaberrations in osteosarcoma by high-definition oligonucleotide array comparative genomic hybridization.

Cancer Genet. Cytogenet. 179:52-61(2007)

 

PubMed=19160414; DOI=10.1002/jcp.21667

Di Fiore R., Santulli A., Drago-Ferrante R., Giuliano M., De Blasio A., Messina C., Pirozzi G., Tirino V., Tesoriere G., Vento R.

Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-amino treatment.

J. Cell. Physiol. 219:301-313(2009)

 

PubMed=19363654; DOI=10.1007/s10585-009-9259-6

Su Y.-X., Luo X.-J., He B.-C., Wang Y., Chen L., Zuo G.-W., Liu B., Bi Y., Huang J.-Y., Zhu G.-H., He Y., Kang Q., Luo J.-Y., Shen J.-K., Chen J., Jin X.-Q., Haydon R.C., He T.-C., Luu H.H.

Establishment and characterization of a new highly metastatic human osteosarcoma cell line.

Clin. Exp. Metastasis 26:599-610(2009)

 

PubMed=19787792; DOI=10.1002/gcc.20717

Ottaviano L., Schaefer K.-L., Gajewski M., Huckenbeck W., Baldus S.E., Rogel U., Mackintosh C., de Alava E., Myklebost O., Kresse S.H., Meza-Zepeda L.A., Serra M., Cleton-Jansen A.-M., Hogendoorn P.C.W., Buerger H., Aigner T., Gabbert H.E., Poremba C.

Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.

Genes Chromosomes Cancer 49:40-51(2010)