MCF-7

MCF7/MCF7细胞系/MCF7细胞株/MCF7人乳腺癌细胞

Cell line name MCF-7

Synonyms MCF 7; MCF.7; MCF7; Michigan Cancer Foundation-7; ssMCF-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7

Accession CVCL_0031

Resource Identification Initiative To cite this cell line use: MCF-7 (RRID:CVCL_0031)

Comments Group: Space-flown cell line (cellonaut).

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: ENCODE project common cell types; tier 2.

Part of: JFCR39 cancer cell line panel.

Part of: JFCR45 cancer cell line panel.

Part of: JWGray breast cancer cell line panel.

Part of: ICBP43 breast cancer cell line panel.

Part of: KuDOS 95 cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: NCI-60 cancer cell line panel.

Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12584.

Registration: Chiron Master Culture Collection; CMCC 10377 (CMCC #10377).

Population: Caucasian.

Doubling time: 1.8 days (PubMed=9671407); 80 hours (PubMed=25984343); 24 hours (PubMed=9488600); 31.2 hours (PubMed=22628656); 25.3 hours (PubMed=24389870); 24.4 hours (PubMed=34238275); ~50 hours (Note=With a range of 30-72 hours) (DSMZ=ACC-115); 25.4 hours (NCI-DTP=MCF7); ~38 hours (PBCF); 56.47 hours (JWGray panel).

Microsatellite instability: Stable (MSS) (PubMed=12661003; PubMed=23671654; Sanger).

Omics: Array-based CGH.

Omics: CTCF ChIP-seq epigenome analysis.

Omics: H2BK120ub ChIP-seq epigenome analysis.

Omics: H3K23ac ChIP-seq epigenome analysis.

Omics: H3K27ac ChIP-seq epigenome analysis.

Omics: H3K27me3 ChIP-seq epigenome analysis.

Omics: H3K36me3 ChIP-seq epigenome analysis.

Omics: H3K4me1 ChIP-seq epigenome analysis.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: H3K79me2 ChIP-seq epigenome analysis.

Omics: H3K9ac ChIP-seq epigenome analysis.

Omics: H3K9me3 ChIP-seq epigenome analysis.

Omics: H4K8ac ChIP-seq epigenome analysis.

Omics: CNV analysis.

Omics: CRISPR phenotypic screen.

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Fluorescence phenotype profiling.

Omics: Glycoproteome analysis by proteomics.

Omics: lncRNA expression profiling.

Omics: Metabolome analysis.

Omics: miRNA expression profiling.

Omics: Myristoylated proteins analysis by proteomics.

Omics: N-glycan profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Omics: Transcriptome analysis by serial analysis of gene expression (SAGE).

Omics: Transcriptome analysis by single cell RNAseq.

Omics: Virome analysis using proteomics.

Anecdotal: This is the first hormone-responsive breast cancer cell line to have been established.

Anecdotal: Have been flown in space on Foton-12 to study cytoskeleton architecture in microgravity (PubMed=11292682; PubMed=15002416).

Anecdotal: Used in a study utilising the fruit fly's olfactory system to detect cancer cells (PubMed=24389870).

Donor information: Helen Marion Mallon (sister Catherine Frances), the patient from which this cell line is derived was a nun at the Immaculate Heart of Mary convent in Monroe, Michigan (CelloPub=CLPUB00336).

Misspelling: MFC-7; Note=Occasionally.

Misspelling: MFC7; Note=Occasionally.

Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.

PubMed=19593635; DOI=10.1007/s10549-009-0460-8

Hollestelle A., Nagel J.H.A., Smid M., Lam S., Elstrodt F., Wasielewski M., Ng S.S., French P.J., Peeters J.K., Rozendaal M.J., Riaz M., Koopman D.G., ten Hagen T.L.M., de Leeuw B.H.C.G.M., Zwarthoff E.C., Teunisse A., van der Spek P.J., Klijn J.G.M., Dinjens W.N.M., Ethier S.P., Clevers H.C., Jochemsen A.G., den Bakker M.A., Foekens J.A., Martens J.W.M., Schutte M.

Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines.

Breast Cancer Res. Treat. 121:53-64(2010)

 

PubMed=20070913; DOI=10.1186/1471-2407-10-15; PMCID=PMC2836299

Tsuji K., Kawauchi S., Saito S., Furuya T., Ikemoto K., Nakao M., Yamamoto S., Oka M., Hirano T., Sasaki K.

Breast cancer cell lines carry cell line-specific genomic alterations that are distinct from aberrations in breast cancer tissues: comparison of the CGH profiles between cancer cell lines and primary cancer tissues.

BMC Cancer 10:15.1-15.10(2010)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

 

DOI=10.4172/2157-7145.S2-005

Fang R.-X., Shewale J.G., Nguyen V.T., Cardoso H., Swerdel M.R., Hart R.P., Furtado M.R.

STR profiling of human cell lines: challenges and possible solutions to the growing problem.

J. Forensic Res. 2 Suppl. 2:5-5(2011)

 

PubMed=21247443; DOI=10.1186/gb-2011-12-1-r6; PMCID=PMC3091304

Edgren H., Murumagi A., Kangaspeska S., Nicorici D., Hongisto V., Kleivi K., Rye I.H., Nyberg S., Wolf M., Borresen-Dale A.-L., Kallioniemi O.-P.

Identification of fusion genes in breast cancer by paired-end RNA-sequencing.

Genome Biol. 12:R6.1-R6.13(2011)

 

PubMed=21378333

Ford C.H.J., Al-Bader M., Al-Ayadhi B., Francis I.

Reassessment of estrogen receptor expression in human breast cancer cell lines.

Anticancer Res. 31:521-527(2011)