Neuro-2a(N2a)

Neuro-2a/Neuro-2a细胞系/Neuro-2a细胞株/Neuro-2a脑神经瘤细胞

Cell line name Neuro-2a

Synonyms NEURO-2A; Neuro 2a; Neuro2a; Neuro2A; N-2a; N2a; N2A; Nb2a; NB2a

Accession CVCL_0470

Resource Identification Initiative To cite this cell line use: Neuro-2a (RRID:CVCL_0470)

Comments Characteristics: Capable of differentiating into various types of neurons.

Doubling time: ~70 hours (DSMZ=ACC-148).

Omics: Deep proteome analysis.

Omics: SNP array analysis.

Miscellaneous: STR profile from personal communication of Rodriguez-Tarduchy Segovia, Gemma.

Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.

Cell type: Neural crest derived neuroblast; CL=CL_0002676.

Disease Mouse neuroblastoma (NCIt: C21993)

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: A/J.

Hierarchy Parent: CVCL_4343 (C-1300)

Children:

CVCL_B7VJ (Abcam Neuro-2a Arc KO) CVCL_6246 (AGN2a) CVCL_4510 (C1300 CLONE NA)

CVCL_C5RF (N2a #34) CVCL_C5RG (N2a #58) CVCL_ZM10 (N2A FLAG-Cdh1)

CVCL_A0TK (N2A Psen1/Psen2 dKO) CVCL_WI11 (N2a-R33) CVCL_WI08 (N2a/Gary)

CVCL_D649 (N2aEGFP) CVCL_D650 (N2aSW) CVCL_RW97 (Na 42/13)

CVCL_B436 (NB2a/d1) CVCL_DF49 (Neuro-2a NucLight Green) CVCL_DF50 (Neuro-2a NucLight Red)

CVCL_K046 (Neuro-2a-Luc) CVCL_C9FD (Neuro-2a/Cas9-Rosa26-Hyg) CVCL_C9FE (Neuro-2a/Cas9-Rosa26-Neo)

CVCL_C9FC (Neuro-2a/Cas9-Rosa26-Puro) CVCL_A9J5 (Neuro-2a/CMV-Luc) CVCL_C9FB (Neuro-2a/HF-Cas9-Rosa26)

CVCL_C9FF (Neuro-2a/LoxP-Cas9-hyg-Rosa26) CVCL_F275 (Neuro-2aTG) CVCL_D3XR (Neuro2a-RXR)

CVCL_E1GT (Neuro2A[TRb1]) CVCL_6268 (ScN2a) CVCL_E0ZD (Ubigene N2A Aldh2 KO)

Sex of cell Male

Category Cancer cell line

STR profile Source(s): Direct_author_submission

 

Markers:

Mouse STR 1-1 11

Mouse STR 1-2 17,18

Mouse STR 2-1 16

Mouse STR 3-2 12,13,14

Mouse STR 4-2 21.3,22.3

Mouse STR 5-5 15,17

Mouse STR 6-4 18,20

Mouse STR 6-7 12

Mouse STR 7-1 25.2

Mouse STR 8-1 16,17

Mouse STR 11-2 15

Mouse STR 12-1 16

Mouse STR 13-1 16.2,17.2

Mouse STR 15-3 21.3,22.3,23.3

Mouse STR 17-2 16

Mouse STR 18-3 22,23

Mouse STR 19-2 12

Mouse STR X-1 26,27

 

Run an STR similarity search on this cell line

Publications

PubMed=11470893; DOI=10.1073/pnas.151242598; PMCID=PMC55414

Enari M., Flechsig E., Weissmann C.

Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody.

Proc. Natl. Acad. Sci. U.S.A. 98:9295-9299(2001)

 

PubMed=14504404; DOI=10.1073/pnas.1834432100; PMCID=PMC208815

Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.

A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions.

Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003)

 

PubMed=19903493; DOI=10.1016/j.jneumeth.2009.11.004

Tremblay R.G., Sikorska M., Sandhu J.K., Lanthier P., Ribecco-Lutkiewicz M., Bani-Yaghoub M.

Differentiation of mouse Neuro 2A cells into dopamine neurons.

J. Neurosci. Methods 186:60-67(2010)

 

PubMed=25193168; DOI=10.1074/mcp.M113.037291; PMCID=PMC4256493

Hornburg D., Drepper C., Butter F., Meissner F., Sendtner M., Mann M.

Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.

Mol. Cell. Proteomics 13:3410-3420(2014)

 

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847.1-847.11(2014)

 

PubMed=25565633; DOI=10.1371/journal.pone.0117154; PMCID=PMC4286239

Oelschlegel A.M., Geissen M., Lenk M., Riebe R., Angermann M., Schaetzl H., Groschup M.H.

A bovine cell line that can be infected by natural sheep scrapie prions.

PLoS ONE 10:E0117154-E0117154(2015)