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D310 - n-Dodecyl-β-D-Maltopyranoside, Anagrade
Catalog # D310
n-Dodecyl-β-D-Maltoside • Lauryl Maltoside • Dodecyl 4-O-α-D-Glucopyranosyl-β-D-Glucopyranoside • DDM / LM
Molecular Formula: C24H46O11
CAS Number: [69227-93-6]
Formula Weight: 510.6
CMC: (H2O) ~ 0.17 mM(1) (0.0087%)
CMC: (0.2M NaCl) ~ 0.12 mM(2)
Aggregation Number: (H2O) ~ 78-149(1,2)
dn/dc: 0.1435ml/gm(4)
Micelle Size: 72 kDa(5)
Purity: ≥ 99% β+α (by HPLC analysis)
Percent Anomer: < 2% α (HPLC)
Percent Alcohol: < 0.005 Dodecanol (HPLC)
pH: 5-8 (1% solution in water)
Solubility: ≥ 20% (in water at 0-5°C)
Conductance: < 40 μS (10% solution in water)
Fluorescence: <10% (0.1% solution in water at 345nm)
Absorbance of a 1% solution in water
225 nm: < 0.1
260 nm: < 0.06
280 nm: < 0.04
340 nm: < 0.02
D322S - n-Decyl-β-D-Maltopyranoside, Sol-Grade
Catalog # D322S
n-Decyl-β-D-Maltoside • Decyl Maltoside • DM
Molecular Formula: C22H42O11
CAS Number: [82494-09-5]
Formula Weight: 482.6
CMC: (H2O) ~ 1.8 mM(1) (0.087%)
CMC: (0.15M NaCl) ~ 1.8 mM(2)
Aggregation Number: (H2O) ~ 69(2)
dn/dc: 0.1473 mL/gm
Purity: ≥ 97% β+α (by HPLC analysis)
Percent Anomer: < 5% α (HPLC)
Percent Alcohol: < 0.05 Decanol (HPLC)
pH: 4-9 (1% solution in water)
Solubility: ≥ 20% (in water at 20°C)
Conductance: < 100 μS (10% solution in water)
Absorbance of a 1% solution in water
225 nm: < 0.2
260 nm: < 0.1
280 nm: < 0.1
340 nm: < 0.05
NG310 - Lauryl Maltose Neopentyl Glycol
Catalog # NG310
2,2-didecylpropane-1,3-bis-β-D-maltopyranoside • LMNG • MNG - DDM
Molecular Formula: C47H88O22
CAS Number: [1257852-96-2]
Formula Weight: 1005.19
Storage Conditions: -20°C
CMC: (H2O) ~ 0.01mM(1) (0.001%)
Purity: ≥98% all anomers (by HPLC analysis)
Percent Anomer: ≤ 4% anomers other than 1,3-bis-β (HPLC)
pH: 5-8 (1% solution in water)
Solubility: ≥ 5% (in water at 20°C)
Conductance: < 100 μS (5% solution in water)
Absorbance of a 1% solution in water
260 nm: ≤ 0.15
280 nm: ≤ 0.12
340 nm: ≤ 0.1
CH210 - Cholesteryl Hemisuccinate Tris Salt
Catalog # CH210
CHS
Molecular Formula: C31H50O4 · C4H11NO3
CAS Number: [102601-49-0]
Formula Weight: 607.9
Identity: IR spectrum confirms to specification
Solubility: 1.2% in a 6% water solution of CHAPS.
Appearance: White powder.
GDN101 - GDN
Catalog # GDN101
Synthetic drop-in substitute for Digitonin • glyco-diosgenin
Molecular Formula: C56H92O25
CAS Number: [1402423-29-3]
Formula Weight: 1165.31
CMC: (H2O) ~ 18 μM(1) (0.0021%)
Purity: ≥ 97% (by HPLC analysis)
pH: 5-8 (1% solution in water)
Solubility: ≥ 10% (in water at 20°C)
Conductance: < 100 μS (10% solution in water)
A835 - Amphipol A8-35
Catalog # A835
Amphipathic surfactant for maintaining solubilized membrane proteins in detergent-free solutions
Molecular Formula: (C6.2H10.3O1.35N0.65Na0.35)~72
CAS Number: [326856-53-5]
Formula Weight: ~ 9 kDa
Solubility: up to 20% in water
Appearance: White Solid
D360S - n-Dodecyl-N,N-Dimethylamine-N-Oxide, Sol-Grade
Catalog # D360S
Lauryldimethylamine-N-Oxide • LDAO • DDAO • N,N-Dimethyl-1-Dodecanamine-N-Oxide
Molecular Formula: C14H31NO
CAS Number: [1643-20-5]
Formula Weight: 229.4
CMC: (H2O) ~ 1-2 mM(5) (0.023%)
CMC: (0.1M NaCl) ~ 0.14 mM(5)
Aggregation Number: (H2O)(6)~ 76
dn/dc: (H2O)0.1381 ml/gm(7)
Micelle Size: 17 kDa, 21.5 kDa (5,8)
Purity: ≥ 95% by HPLC analysis
pH: 5-9 (1% solution in water)
Solubility: ≥ 30% (in water at 20°C)
Conductance: < 500 μS (10% solution water)
Peroxide: < 500 μM
Appearance: white solid
Absorbance of a 1% solution in water
260 nm: < 0.2
280 nm: < 0.1
340 nm: < 0.1
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文献和实验本身不带标签，则需要使用离子交换层析。 离子交换层析 离子交换层析推荐使用 RESOURCE 和 Capto HiRes 系列，能得到比较好的分辨率。不过要注意阴离子交换实验中不要添加阴离子型去垢剂，阳离子交换实验中则不能加入阳离子型去垢剂，以免影响层析柱载量。 离子交换之前需降低缓冲液中盐浓度，由于膜蛋白的特殊性，推荐使用脱盐柱进行此步骤，根据样品体积可选择 HiTrap Desalting 5 mL 或 HiPrep 26/10 Desalting 53 mL 两种常用柱型。最终上样体积不要超过
交换 亲和层析之后，往往使用分子筛做最后的精纯，但如果杂质依然很多或者膜蛋白本身不带标签，则需要使用离子交换层析。 离子交换层析 离子交换层析推荐使用 RESOURCE 和 Capto HiRes 系列，能得到比较好的分辨率。不过要注意阴离子交换实验中不要添加阴离子型去垢剂，阳离子交换实验中则不能加入阳离子型去垢剂，以免影响层析柱载量。 离子交换之前需降低缓冲液中盐浓度，由于膜蛋白的特殊性，推荐使用脱盐柱进行此步骤，根据样品体积可选择 HiTrap Desalting
液，三个一起上 从以上三个结果可以看到，第一种孵育方法抗原得率最高，第三种抗原得率最低。如果靶蛋白丰度较高，第一种和第二种差别并不显著。注意，整个体系选择同样的孵育顺序，以确保结果的重复性。 5、各种 buffer 整个 co-IP 系统，涉及到裂解液，结合液，洗涤液，洗脱液，这4种溶液要如何考量呢？ 裂解液---样本质量很大程度受限于裂解液，要考虑其中的去垢剂种类。 非变性裂解液，含有非离子型去垢剂，有助于稳定天然蛋白构象，比如 IP lysis buffer。不建议使用变性裂解
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