Norgen Biotec热门产品

Total RNA Purification Kits

SKU 17200

Overview

Extract high quality & quantity total RNA including miRNA

No phenol step required; isolate all RNA in one fraction

Bind & elute all RNA irrespective of size or GC content, without bias

Very sensitive & linear down to a few cells without the need for carrier RNA

Isolate from a wide variety of specimens

Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information 

on Norgen's NGS services

Available in a variety of formats to suit your needs

Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily 

fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and

 A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all 

sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. 

Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the

 column binds small RNAs regardless of their GC content.

 

Plasma/Serum Cell-Free Circulating DNA Purification Kits

SKU 55500

Overview

Isolate all sizes of circulating DNA from plasma and serum samples

Isolate viral and bacterial DNA

Versatile plasma and serum input volumes

Concentrate circulating DNA into a flexible elution volume

Isolate inhibitor-free cell-free circulating DNA

Compatible with Streck Cell-Free DNA BCT® Tubes

Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a fast, reliable and convenient spin column method for the isolation of high quality, high purity and inhibitor-free

 cell-free circulating DNA (cfc-DNA) from plasma/serum sample volumes. The purified plasma/serum cfc-DNA is fully compatible

 with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Background

Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states

 as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers

 for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free

 mitochondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short

 fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal 

diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders, 

screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.

Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit

Ideal for handling initial plasma/serum volumes ranging from 10 μL up to 200 μL. The kit is designed to isolate all sizes of 

cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume

 ranging from 25 µL to 50 µL.

Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit

Ideal for handling initial plasma/serum volumes ranging from 200 μL up to 500 μL. The kit is designed to isolate all sizes of

 cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume 

ranging from 25 µL to 50 µL.

Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit

Ideal for handling initial plasma/serum volumes ranging from 1 mL - 4 mL. The first column will handle the large volume

 input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL

Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit

Ideal for handling initial plasma/serum volumes ranging from 5 mL- 10 mL. The first column will handle the large volume

 input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL

 

Exosomal RNA Isolation Kit

SKU 58000

Overview

Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA

Bind and elute all RNA irrespective of size or GC content, without bias

No phenol extractions

No Proteinase K treatment

No carrier RNA

Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL

Purify high-quality RNA in 15-20 minutes

Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information

 on Norgen's NGS services

Purification is based on spin column chromatography that uses Norgen’s proprietary resin

This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified

 exosomes. It allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or

 plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.

The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. Moreover, the kit allows the user 

to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be 

used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase

 protection and primer extension, and expression array assays.

 

Single Cell RNA Purification Kit

SKU 51800

Overview

Fast and easy processing using rapid micro spin column format

Small elution volume of 8µL ready for direct utilization in qRT-PCR reactions

Isolate total RNA including microRNA in a concentrated ready-to-use format

Isolate high quality total RNA from a variety of sources

RNA can be isolated from a single cell to 100,000 cells

Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Very sensitive kit designed to work with as low as a single cell and up to 100,000 cells. The special design of the micro 

spin-column allows a small elution volume of as little as 8 µL.

Rapid 15 minute method for the isolation and purification of total RNA (including miRNA) from small input amounts of cultured

 animal cells, CTC, stem cells, immuno-sorted cells, and microdissected samples including laser-capture microdissection (LCM). 

The purified RNA is of the highest purity and integrity, and can be used in a number of qRT-PCR and digital PCR and other

 whole transcriptome applications.

 

cf-DNAcf-RNA Preservative Tubes

SKU 63950

Overview

Preservation and isolation of both cf-DNA and cf-RNA from a single tube

Fixative-free preservative, no cross-linking of DNA

Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C

Preserve cf-RNA for 30 days at ambient temperature

Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature

No plasma volume loss after shipping/transportation

Prevent hemolysis allowing better separation of plasma

Prevent apoptosis of blood cells and fragmentation of genomic DNA

Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA

Vacuumed to draw 8.7 mL of blood in 10 mL tubes

The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019

Norgen's cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of

 cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and

 shipping. Using these tubes in combination with Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or 

Norgen's Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free

 cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen's cf-DNA/cf-RNA Preservative 

Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and 

cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, 

Southern Blot analysis, gene expression analysis, microarrays and NGS.

This product is for research use only and not for use in diagnostic procedures.