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All-In-One 5X RT MasterMix with gDNA Removal
一步法反转MasterMix试剂盒，现货销售
G592
100 rxn
All-In-One 5X RT MasterMix with gDNA Removal is a convenient and ready-to-use formulation for first-strand cDNA synthesis,
including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and
this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will
be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript Hot Reverse
Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of
Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream
applications.®
Product Features:
Easy one-step setup reduces pipetting and sample handling
High cDNA yields from difficult samples with OneScript Hot Reverse Transcriptase (Cat. No. G593®)
Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
Removes contaminating gDNA with temperature-sensitive DNase
Also includes dNTPs, Oligo (dT)s, and random primers
Earn 2X the rewards points
All-In-One 5X RT MasterMix with gDNA Removal is a convenient and ready-to-use formulation for first-strand cDNA synthesis,
including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and
this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will
be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript Hot Reverse
Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo
(dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream
applications.®
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文献和实验Real-time qPCR 手册——手把手教你从菜鸟到高手
尽量靠在上游引物； 长度 30-45 bp，Tm 比引物高至少 5 ℃； 5』端不要是 G，G 会有淬灭作用，影响定量 四、Real-time qPCR 操作过程 1. RNA 提取和反转录 在抽提 RNA 过程中任一环节的不正确操作都可能导致 RNA 酶的污染。由于 RNA 酶的活性很难完全抑制，预防其污染是十分必要的。在实际的操作中应遵循下面一些原则： 1. 全程佩戴一次性手套。皮肤经常带有细菌和霉菌，可能污染 RNA 的抽提并成为 RNA 酶的来源。培养良好
的，PAGE胶纯化的引物在这里不适用）作为逆转录引物合成cDNA。由于引物中带有T7序列，因此合成的cDNA的3'端也带有T7序列。选用Affymetrix公司提供的Enzo BioArray HighYield RNA Transcript Labeling Kit（Cat. No. 900182，10次标记），通过试剂盒提供的生物素标记的核糖核酸和T7 RNA聚合酶，以体外转录（IVT）的方式扩增并标记cRNA探针。试剂盒提供体外转录和标记的所有试剂，可以作10个反应。标记好的探针经过随机片断
Kit(Cat.No.900182,10次标记)，通过试剂盒提供的生物素标记的核糖核酸和T7 RNA聚合酶，以体外转录（IVT）的方式扩增并标记cRNA探针。试剂盒提供体外转录和标记的所有试剂，可以作10个反应。标记好的探针经过随机片断化（fragmentation，试剂自行配制）后得到大小约为35-200个碱基大小的RNA片断即可和芯片进行杂交。 2）Affymetrix公司的Genotyping（如人类SNP图谱分析芯片）和健康研究芯片： 这一类芯片用于检测样品基因组的变异，因而只需纯化基因
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