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T25
Pan02细胞系;Pan02细胞株;小鼠胰腺癌细胞,Pan02;小鼠胰腺癌细胞,
Pan02 Cell line name Panc02 Synonyms Panc-02; Panc 02; Pan02; Pan-02; PAN 02; Panc02-H0 Accession CVCL_D627 Resource Identification Initiative To cite this cell line use: Panc02 (RRID:CVCL_D627) Comments Transformant: ChEBI; CHEBI:34342; 3-methylcholanthrene (3-MC; 20-methylcholanthrene; 20-MC; MCA). Derived from site: In situ; Pancreas; UBERON=UBERON_0001264. Disease Mouse pancreatic ductal adenocarcinoma (NCIt: C34041) Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090) Breed/subspecies: C57BL/6. Hierarchy Children: CVCL_D7FV (Pan-02-Luc) CVCL_D628 (Panc02-H7) CVCL_A8QL (Panc02-luc) CVCL_E1AG (Ubigene Panc02 Bhlhe40 KO) CVCL_E1AH (Ubigene Panc02 Cd44 KO) Sex of cell Male Category Cancer cell line STR profile Source(s): Ubigene=YC-C067 Markers: Mouse STR 4-2 20.3 Mouse STR 5-5 16,18 Mouse STR 6-4 18 Mouse STR 6-7 17 Mouse STR 12-1 17 Mouse STR 15-3 22.3 Mouse STR 18-3 15 Mouse STR X-1 29 Run an STR similarity search on this cell line Publications PubMed=6692374 Corbett T.H., Roberts B.J., Leopold W.R., Peckham J.C., Wilkoff L.J., Griswold D.P. Jr., Schabel F.M. Jr. Induction and chemotherapeutic response of two transplantable ductal adenocarcinomas of the pancreas in C57BL/6 mice. Cancer Res. 44:717-726(1984) PubMed=11558631; DOI=10.1385/IJGC:29:1:37 Wang B.-L., Shi Q., Abbruzzese J.L., Xiong Q.-H., Le X.-D., Xie K.-P. A novel, clinically relevant animal model of metastatic pancreatic adenocarcinoma biology and therapy. Int. J. Pancreatol. 29:37-46(2001) PubMed=20805420; DOI=10.4049/jimmunol.0902609; PMCID=PMC3693576 Gnerlich J.L., Mitchem J.B., Weir J.S., Sankpal N.V., Kashiwagi H., Belt B.A., Porembka M.R., Herndon J.M., Eberlein T.J., Goedegebuure P., Linehan D.C. Induction of Th17 cells in the tumor microenvironment improves survival in a murine model of pancreatic cancer. J. Immunol. 185:4063-4071(2010) PubMed=21720167; DOI=10.1159/000329413 Partecke L.I., Sendler M., Kaeding A., Weiss F.U., Mayerle J.V., Dummer A., Nguyen T.D., Albers N., Speerforck S., Lerch M.M., Heidecke C.-D., von Bernstorff W., Stier A. A syngeneic orthotopic murine model of pancreatic adenocarcinoma in the C57/BL6 mouse using the Panc02 and 6606PDA cell lines. Eur. Surg. Res. 47:98-107(2011) PubMed=22208613; DOI=10.2174/156652412799218868; PMCID=PMC3799885 Wang Y., Zhang Y., Yang J., Ni X., Liu S., Li Z., Hodges S.E., Fisher W.E., Brunicardi F.C., Gibbs R.A., Gingras M.-C., Li M. Genomic sequencing of key genes in mouse pancreatic cancer cells. Curr. Mol. Med. 12:331-341(2012)
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文献和实验*发表【中文论文】请标注:由上海酶研生物科技有限公司提供;
*发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
流式细胞术样本制备
Pyrrolizidine alkaloid clivorine inhibits human normal liver L-02 cells growth and activates p38 mitogen-activated protein kinase in L-02 cellsAbstractClivorine, a pyrrolizidine alkaloid extracted from Chinese medicinal plant Ligularia hodgsonii
HePG2细胞属人肝肿瘤的恒生细胞株,L-02细胞则是人胎肝细胞株,二者所使用的培养基可以是一样的,即最常见的DMEM。一干使用低糖的。 细胞长满培养瓶时既要传代。使用胰酶消化即可。用D-Hank's液配制成0.25%的胰酶。使用前37度预热,细胞经PBS清洗两遍后,每个中号培养瓶加0.7ml的胰酶,胰酶的量可根据自己培养瓶的大小而定,原则就是能够盖满瓶底。加入胰酶以后,为使酶的活性最大,将培养瓶盖拧紧后放回培养箱中,3分钟左右即用含血清的培养基终止消化。如果在室温情况下消化,时间相应
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